Electron Microscopes


Information on Electron Microscopes - Advantages, disadvantages etc.
Jessica Phillips
Mind Map by Jessica Phillips, updated more than 1 year ago
Jessica Phillips
Created by Jessica Phillips over 9 years ago

Resource summary

Electron Microscopes


  1. Advantages
    1. Higher resolution
      1. Higher magnification
        1. Fine detail can be observed
          1. SEM can provide 3D image
          2. Disadvantages
            1. Specimen is dead
              1. Natural colours can not be seem
                1. Specimen needs to be dehydrated
                  1. Expensive
                    1. Not portable
                      1. Technical expertise required
                        1. There is likely to be artifacts


                          • Artifact - A structure that is not part of the specimen.
                          1. Heat is generated
                            1. Vacuum pump required
                              1. Specimen and electrons need to be contained
                              2. There are two types of electron microscopes
                                1. Scanning Electon Microscopes
                                  1. Images are 3D
                                    1. Depth of field
                                      1. Internal structures can't be seen
                                      2. Resolution = 0.5 nm
                                        1. Cannot achieve same resolution as TEM
                                        2. Magnification = x 100,000
                                        3. Transmission Electron Microscopes
                                          1. Images are 2D
                                            1. Internal structures can be seen
                                              1. Resolution = 0.1 nm
                                                1. Magnification = x 500,000
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