Light microscopes

Description

Light Microscopes - Advantages, disadvantages etc.
Jessica Phillips
Mind Map by Jessica Phillips, updated more than 1 year ago
Jessica Phillips
Created by Jessica Phillips over 9 years ago
354
16

Resource summary

Light microscopes

Attachments:

  1. Advantages
    1. No vacuum
      1. Specimen can be dead or alive
        1. Low cost
          1. Good for use in schools etc.
          2. Portable
            1. Less likely to produce atifacts
              1. Natural colours seen
                1. Preparation of specimen
                  1. Less harsh than for EM
                  2. No technical expertise required
                    1. Easy to use
                  3. Disadvantages
                    1. Low magnification
                      1. Magnification - X1500 to X2000
                      2. Low resolution
                        1. Can't produce 3D images
                          1. Not as detailed as electron microscopes
                          2. Improvements to resolution
                            1. Having an objective lens with a short vocal length
                              1. Having a film of immersion oil between the objective lens and the specimen on the slide
                              2. Staining

                                Annotations:

                                • Stains are coloured chemicals used to distinguish (highlight) specific structures.
                                • The first known stain was saffron.
                                1. Coloured stains
                                  1. Stains that bind to specific chemicals on or in the specimen allowing it to be seen
                                  2. Differential stains
                                    1. Involves more than one stain on a specimen
                                      1. Used for contrast
                                    Show full summary Hide full summary

                                    Similar

                                    Function and Structure of DNA
                                    Elena Cade
                                    AQA Biology 3.3 Structure of an epithelial cell
                                    Charlotte Hewson
                                    AQA Biology 12.1 cellular organisation
                                    Charlotte Hewson
                                    AS Biology Unit 1
                                    lilli.atkin
                                    AQA Biology 11.2 mitosis
                                    Charlotte Hewson
                                    AQA Biology 11.1 replication of DNA
                                    Charlotte Hewson
                                    Cell Transport
                                    Elena Cade
                                    Haemoglobin
                                    Elena Cade
                                    Biological Definitions
                                    Yamminnnn
                                    AQA AS Biology Unit 2 DNA and Meiosis
                                    elliedee
                                    The Digestive system
                                    Elena Cade