diaggregation of explants

Description

Flowchart on diaggregation of explants, created by Isabell Rupa on 03/01/2018.
Isabell Rupa
Flowchart by Isabell Rupa, updated more than 1 year ago
Isabell Rupa
Created by Isabell Rupa almost 7 years ago
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Resource summary

Flowchart nodes

  • Selection and isolation of organ
  • Dissection and removal of necrotic cells and fat cells
  • Mechanical dissagregation
  • Enzymatic dissagregation
  • Primary expant technique
  • Chopping and slicing of tissue, sieving, syringing, pippeting, etc.
  • Trypsin 
  • Collagenase
  • Other enzymes ( bacterial proteases)
  • Explant
  • Warm Trypsinization
  • Cold Trypsinization
  • Resuspended and seed
  • Primary culture 
  • sub-culture 
  • Cell line
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