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diaggregation of explants
Description
Flowchart on diaggregation of explants, created by Isabell Rupa on 03/01/2018.
Flowchart by
Isabell Rupa
, updated more than 1 year ago
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Created by
Isabell Rupa
about 7 years ago
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Resource summary
Flowchart nodes
Selection and isolation of organ
Dissection and removal of necrotic cells and fat cells
Mechanical dissagregation
Enzymatic dissagregation
Primary expant technique
Chopping and slicing of tissue, sieving, syringing, pippeting, etc.
Trypsin
Collagenase
Other enzymes ( bacterial proteases)
Explant
Warm Trypsinization
Cold Trypsinization
Resuspended and seed
Primary culture
sub-culture
Cell line
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