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11939435
Nitritos en Embutidos
Description
Diagrama para realizar analisis de nitritos en embutidos mediante Reactivos de Griess
Flowchart by
Ana Puga
, updated more than 1 year ago
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Created by
Ana Puga
almost 7 years ago
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Resource summary
Flowchart nodes
NITRITOS
Pesar 2 grs en 2 vasos de precipitado chicos
Vaso Preciptado (binary/octet-stream)
Deshacer grumos con 100 ml agua de nitritos en matraz de 250ml
Dejar 3hrs en baño maria y atemperar
Matraz Aforado 500ml (binary/octet-stream)
Añadir 5ml de HgCl2 si hay color y aforar a 250ml
Filtrar 30ml de cada repeticion con papel filtro y embudo
Tomar alicuota de 15ml a matraz de 50 ml aforado y aforar con agua de nitritos
Cloruro D Emercurio (binary/octet-stream)
Matraz Aforado 500ml (binary/octet-stream)
Añadir 1 ml de Reactivo Griess 1 y 1 de Reactivo 2 a cada matraz
Dejar actuar reactivos 20 min. Encender espectrofotometro a 520 nm.
Colocar blanco y "tarar" espectro, enseguida realizar lecturas de las repeticiones
Realizar un blanco al mismo tiempo con pura agua de nitritos
Realizar calculos
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