Maxam and Gilbert

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BSc degree Bioinformatics Note on Maxam and Gilbert , created by yolajoy on 02/04/2014.
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Note by yolajoy, updated more than 1 year ago
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Created by yolajoy over 10 years ago
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hypothetical sequence with cleavage points indicated

the

the sequence of the DNA fragment can be indentified but using the gel electrophoresis if the same sized fragment appears in C only and T and C or G only and A and G you can assume that the base modified here is a C or a G whereas if a specifically sized fragment appears in with T and C or A and G you can assume that the base there was either a T or an A So by reading through the electrophoresis we can see which base is in each position along the sequence.

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