DISAGGREGATION OF PLANTS

Description

Flowchart on DISAGGREGATION OF PLANTS, created by Rashedha Camila on 27/12/2017.
Rashedha Camila
Flowchart by Rashedha Camila , updated more than 1 year ago
Rashedha Camila
Created by Rashedha Camila about 7 years ago
20
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Resource summary

Flowchart nodes

  • DISAGGREGATION OF PLANTS
  • PRIMARY AGGREGATION
  • MECHANICAL DISAGGREGATION
  • ENZYMATIC DISAGGREGATION
  • ​​​​DISSECTION
  • FINE CHOPPING  
  • WASH BY SETTLING
  • TRANSFER IN LAST WASH
  • REMOVE BSS
  • ADD THIN FILM OF MEDIUM
  • INCUBATE FOR 2-3 WEEKS
  • EXPLANT OUTGROWTHS
  • TISSUE SAMPLE
  • CELL TISSUE + DEBRIS
  • MORE CELLS + TISSUE DEBRIS
  • VIABLE CELLS + TISSUE DEBRIS
  • VIABLE CELLS + DAMAGED CELLS
  • PRIMARY ADHERENT CELL CULTURE
  • BIOPSY IN MEDIUM + ANTIBIOTIC
  • DISSECTION IN BSS  + ANTIBIOTIC
  • CHOPPED TISSUE
  • COMPLETE MEDIUM + COLLAGENASE
  • DISAGGREGATED TISSUE + TISSUE RESIDUES
  • DISPERSED CELLS + RESIDUAL CULTURE OF EPITHELIAL ELLS
  • DISPERSED CELL SUSPENSION CONTAINS ENRICHED FIBROBLASTS
  • PRIMARY CELL CULTURE
  • ENRICHED EPITHELIAL FRACTION
  • PRIMARY CELL CULTURE
  • EDTA TREATMENT
  • COLLAGENSE
  • CENTRIFUGE
  • RESUSPEND
  • WASH
  • PRIMARY CELL CULTURE
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