Cell Line Traits

Question	Answer
Primary/Immortal Cells - Anchorage Dependent	Yes
Primary/Immortal Cells - Growth Factor Dependent	Yes
Primary/Immortal Cells - Density Dependent Inhibition	Yes
Primary/Immortal Cells - Morphology	Flat, extended growing surface with elongated stress fiber network
Primary/Immortal Cells - Monolayer Growth	Yes
Density Dependent Inhibition	Cells will stop dividing once in full contact with other cells- forming a monolayer
Growth Factors	Allow signaled proliferation only (found in serum) in normal cells
Transformed Cell Line	Immortalised cell line that could form tumors in the immunocompromised
Transformed Cell Line - Anchorage Dependent	No
Transformed Cell Line - Responds to Apoptic Signals	No
Transformed Cell Line - Growth Factor Dependent	Reduced, but not gone- autocrine stimulation
Autocrine Stimulation	Cell produces chemicals that signal itself
Transformed Cell Line - Density Dependent Inhibition	No- leads to necrosis of cells below surface
Transformed Cell Line - Increased Metabolic Rate	Yes- inc. Glucose transport, lactate production & protein generation
Warburg Effect	Cells metabolise differently- glycolysis & lactic acid fermentation, which is detectable
Transformed Cell Line - Morphology	No defined shape, increased motility, decreased surface fibrinogen
Fibrinogen	Protein that attaches cells to each other & causes density dependent inhibition
Tumerogenic Cell	If injected into healthy mice, these cells will invade & metastasie tissue
E-Cadherin	Also prevents density dependent growth

Resource summary

	Created by gina_evans0312 over 10 years ago