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25833817
Extracción poliaminas
Description
Extracción poliaminas
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extracción poliaminas
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Flowchart by
luis temiño
, updated more than 1 year ago
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Created by
luis temiño
almost 4 years ago
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Resource summary
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INICIO
Pesar 0,5 g de tejido triturado y congelado
Añadir 2,5mL de HClO4 5%
Vortex
Añadir 10 uL de hexanodiamina a 100ppm
Vortex
Reposo 1 hora en hielo
Centrifugar 20 minutos a 4500 rpm y a 4ºC
Pasar el sobrenadante a un tubo Falcon nuevo
Añadir 1,66mL de NaOH 2M
Añadir 10 uL de cloruro de benzoílo
Vortex
Derivatizar: 20 minutos a Tº ambiente
Añadir 3,33 mL de NaCl saturado
Vortex
Añadir 2 mL de dietil-éter
Vortex 1 minuto
Dejar reposar
Extraer el sobrenadante a un tubo nuevo (vidrio)
Eliminar el disolvente con flujo de N2
Resuspender en 130 uL de metanol
Filtrar
Pasar a viales de vidrio de UPLC con inserto
Pinchar 4 uL con el programa 30-50 % ACN 40ºC
FIN
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