Oxidation Practical

Description

Structural Basis for Biological Function (Membrane Proteins) Quiz on Oxidation Practical, created by gina_evans0312 on 05/01/2014.
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Quiz by gina_evans0312, updated more than 1 year ago
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Created by gina_evans0312 almost 10 years ago
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Resource summary

Question 1

Question
What is the difference between Stage 3 & Stage 4?
Answer
  • Stage 3 is between ADP addition and ADP running out
  • Stage 3 is between ADP running out and oxygen running out
  • Stage 4 is between ADP running out and oxygen running out
  • Stage 4 is between O2 running out and ADP running out

Question 2

Question
Measuring oxygen consumption is done by looking at the change in the X co-ordinates
Answer
  • True
  • False

Question 3

Question
Once you've worked out the change in O2 concentraction, how do you calculate nmol O2 /min/mg protein
Answer
  • Divide by mg of protein
  • Divide by 60
  • Divide by O2 consumed

Question 4

Question
Assume a change in the axis of 15mm, and a protein concentration of 1.4mg. Calculate the Rate
Answer
  • 25.7 nm Oxygen/min/mg protein
  • 10.7 nm Oxygen/min/mg protein
  • 50.4 nm Oxygen/min/mg protein

Question 5

Question
What is the equation for the respiratory control ratio?
Answer
  • Rate of phase 4/ Rate of phase 3
  • Rate of phase 3/ Rate of phase 4
  • Rate of phase 3 x Rate of phase 4

Question 6

Question
ADP/O ratio = Moles of ADP added/Moles of ADP consumed
Answer
  • True
  • False

Question 7

Question
To convert from O2 to O, what must you do?
Answer
  • Double it
  • Halve it
  • Square it

Question 8

Question
What must be checked before calculating the ADP/O ratio?
Answer
  • That both sides are in the same molar concentration
  • That the oxygen consumed has been converted into nm
  • That the ratio is positive
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