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Cyt C Oxidase & F1F0

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Structural Basis for Biological Function (Membrane Proteins) Quiz on Cyt C Oxidase & F1F0, created by gina_evans0312 on 18/12/2013.
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Quiz by gina_evans0312, updated more than 1 year ago
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Created by gina_evans0312 over 10 years ago
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Question 1

Question
What is the main role of cytochrome aa3?
Answer
  • Takes e- from ubiquinol and reduces oxygen to water
  • Takes e- from ubiquinol and reduces oxygen to peroxide
  • Takes e- from cytochrome c and reduces oxygen to peroxide
  • Takes e- from cytochrome c and reduces oxygen to water

Question 2

Question
Complex IV contains how many subunits?
Answer
  • 12
  • 13
  • 14
  • 15

Question 3

Question
Complex IV doesn't transfer protons across the inner mitochondrial membrane
Answer
  • True
  • False

Question 4

Question
Which ions does it contain, and how many of each?
Answer
  • 2 Fe (in haeme groups) and 3 Cu (2 as a Cu-Cu binuclear centre, 1 as a binuclear Fe-Cu centre)
  • 2 Cu (in haeme groups) and 3 Fe (2 as a Fe-Fe binuclear centre, 1 as a binuclear Fe-Cu centre)
  • 2 Mg (in haeme groups) and 3 Cu (2 as a Cu-Cu binuclear centre, 1 as a binuclear Fe-Mg centre)

Question 5

Question
Cytochrome aa3 complex consists almost entirely of alpha helices
Answer
  • True
  • False

Question 6

Question
Put the following in order A- Cyt c B- CuA centre C- Cyt a D- Cyt a3-CuB centre E - Water •Transfer from cyt a3-CuB centre to oxygen
Answer
  • A-B-C-D-E
  • A-C-D-B-E
  • A-C-B-D-E

Question 7

Question
How many protons are pumped by complex 4, and how many are used to make water (per Oxygen molecule)?
Answer
  • 4 & 4
  • 4 & 2
  • 2 & 4

Question 8

Question
Which conformation of Complex IV has the low affinity for protons?
Answer
  • Conf. A
  • Conf. B
  • Conf. C

Question 9

Question
Protons bind to Complex IV during Conformation B
Answer
  • True
  • False

Question 10

Question
What happens during conformation B that lowers the Complex IV's affinity for protons?
Answer
  • Energy is put in
  • Energy is lost
  • The protein has an Fe group added

Question 11

Question
No question, just look at it and learn it
Image:
Complex_IV (image/png)
Answer
  • Okay?
  • Done

Question 12

Question
ATP synthase is found on the inner mitochondrial membrane
Answer
  • True
  • False

Question 13

Question
ATP synthase is divided into two sections, F1 & F0 Which is peripheral and which is integral?
Answer
  • F1 is peripheral F0 is integral
  • F0 is peripheral F1 is integral

Question 14

Question
Name the function of ATP synthase
Answer
  • ATP synthesis
  • ATP hydrolysis
  • ATP translocation
  • Proton translocation

Question 15

Question
If ATP is high, and the proton gradient is low, what will Complex V do instead?
Answer
  • Hydrolyse ATP
  • Synthesise GTP
  • Break itself down

Question 16

Question
Protons flow through F1 to power the ATP synthesis in F0
Answer
  • True
  • False

Question 17

Question
Name the subunits of F1
Answer
  • Alpha-Epsilon
  • A-E
  • 1-5

Question 18

Question
Which of the following subunit(s) are the largest in F1?
Answer
  • Alpha & Beta
  • Gamma & Delta
  • Beta and Gamma
  • Alpha and Epsilon

Question 19

Question
What is the role of the delta subunit in F1?
Answer
  • Stabiliser for the F1 head
  • ATP synthesis
  • Connets F1 too F0

Question 20

Question
The epsilon subunit of F0 connects the gamma to the C subunit
Answer
  • True
  • False

Question 21

Question
F0 contains how many subunits?
Answer
  • 2- A & B
  • 3- A, B & C
  • 2- Alpha & Beta

Question 22

Question
The F1 subunit is...
Answer
  • 8nm long
  • 18nm long
  • 12nm long
  • 90nm wide
  • 19nm wide
  • 9nm wide

Question 23

Question
The Y subunit of F1 is made of 2 beta sheets that have been bent, but are bound at the base
Answer
  • True
  • False

Question 24

Question
When the gamma subunit is in contact with a beta subunit, the ATP catalysis site is in it's _ formation
Answer
  • Open
  • Loose
  • Tight

Question 25

Question
A subunit contains ADP + Pi- what conformation is it in?
Answer
  • Loose
  • Tight
  • Open

Question 26

Question
Whilst the subunit is replacing ATP with ADP + Pi, the gamma subunit remains in plance
Answer
  • True
  • False

Question 27

Question
What order do the three conformations work in? Assume you start with the Loose formation
Answer
  • Loose-Tight-Open
  • Loose-Open-Tight

Question 28

Question
State the characteristics of the C subunit of F0
Answer
  • Small
  • Large
  • Hydrophobic
  • Hydrophilic
  • Integral
  • Peripheral

Question 29

Question
How many monomers make up the C subunit?
Answer
  • 8-12
  • 8-10
  • 4-10

Question 30

Question
The C subunit contains 3 rings of monomer
Answer
  • True
  • False

Question 31

Question
Why is there a variance across species of the number of monomers in the C subunit?
Answer
  • The more complex an organism, the more efficient the subunit
  • The more complex an organism, the less efficient the subunit
  • The larger the organism, the more subunits are required
  • Mammals have 8, birds have 12, all lesser animals and prokaryotes have 14 or more

Question 32

Question
More subunits require more protons to move them, so more subunits is less efficient
Answer
  • True
  • False

Question 33

Question
How is the A subunit described?
Answer
  • A proton half channel
  • A proton channel
  • A proton quarter channel

Question 34

Question
Protons passing through the A subunit...
Answer
  • Use it to enter the C subunit and exit to the cytoplasm
  • Use it to exit into C subunit and enter from the cytoplasm

Question 35

Question
Each subunit can hold 2 protons
Answer
  • True
  • False

Question 36

Question
The momevent of the protons around the C subunit powers what?
Answer
  • The movement of the gamma subunit
  • The conformational changes of the beta subunits
  • The attachment of the alpha subunits
  • The rotation of the delta subunit

Question 37

Question
The rotation of the F1 has been proved by attaching a microtubule filament to the F1 head and examining it under the microscope
Answer
  • True
  • False

Question 38

Question
Only the gamma subunit of the F1 complex rotates
Answer
  • True
  • False

Question 39

Question
How is the Alpha/Beta spheroid of F1 held in place?
Answer
  • B1 subunit
  • B2 subunit
  • Epsilon subunit
  • Delta subunit

Question 40

Question
For every _degree rotation, 1 molecule of ATP is produced
Answer
  • 90
  • 120
  • 180
  • 360

Question 41

Question
The mechanism of the gamma subunit causes an affinity change for ATP/ADP + Pi, driving the cycle
Answer
  • True
  • False
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