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Alternative Oxidase

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Structural Basis for Biological Function (Membrane Proteins) Quiz on Alternative Oxidase, created by gina_evans0312 on 18/12/2013.
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Quiz by gina_evans0312, updated more than 1 year ago
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Created by gina_evans0312 over 10 years ago
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Resource summary

Question 1

Question
Why is human african tropanomosis so common?
Answer
  • Most drugs are outdated
  • Most drugs have severe side effects
  • Most drugs are incredibly expensive
  • Most drugs cannot be used on the immunocompromised

Question 2

Question
The cause of Human African Tropanomosis is a virus known as Tropanosome
Answer
  • True
  • False

Question 3

Question
Tropanosomes contain 1 mitochondria that depends on what for energy?
Answer
  • Glycerol-3-Dehydrogenase
  • Succinate Dehydrogenase
  • NADH Dehydrogenase
  • Malate Dehydrogenase

Question 4

Question
The enzyme that tropanosomes rely on allows glycolysis to continue indefinitely
Answer
  • True
  • False

Question 5

Question
What other components make up the tropanonsome's mitochondria?
Answer
  • Alternative Oxidase
  • Quinol
  • Cytochrome C
  • Cytochrome BC1 complex

Question 6

Question
How does Alternative Oxidase get its energy source?
Answer
  • Glucose in the blood plasma
  • Glucose in the infected red blood cell
  • Glucose from other blood proteins

Question 7

Question
Cryptosporidium Parvum can only become fatal to the immunocompromised
Answer
  • True
  • False

Question 8

Question
Name the following
Image:
C.P_Cysts (image/png)
Answer
  • Cryptosporidium parvum cysts
  • Cryptosporidium parvum oocysts
  • Cryptosporidium parvum oocytes

Question 9

Question
Which of the following are characteristic of Alternative Oxidase?
Answer
  • Membrane bound
  • Cytoplasmic
  • Reduces O2 to H2O
  • Reduces O2 to H2O2
  • No absorption about 340nm

Question 10

Question
Alt. Ox. is a 34kDa monomer
Answer
  • True
  • False

Question 11

Question
Why does Alt. Ox. not absorb above 340nm?
Answer
  • It contains no Cyt's or Fe-S
  • It contains no chromosomes
  • It contains no Cty's or Flavins

Question 12

Question
To activate, Alt. Ox must form a dimer
Answer
  • True
  • False

Question 13

Question
What is particular about Rubreythrin?
Answer
  • It is the olden protein yet sequenced - 4 billion years old
  • It's the smallest protein yet sequenced - just 40 aa long
  • It's the largest protein yet sequenced - 400,000 kDa

Question 14

Question
Only 6 aa in Alt. Ox have been conserved, relating it to other di-iron proteins
Answer
  • True
  • False

Question 15

Question
The conserved sequence of Alt. Ox. codes for amino acids holding what?
Answer
  • Iron
  • Magnesium
  • Copper
  • Sodium

Question 16

Question
Which of the following list characteristics of Alt. Oxidase?
Answer
  • Sits horizontally in the membrane
  • Sits vertically in the membrane
  • Made of 2 chains, almost entirely of alpha helices
  • Made of 3 chains, almost entirely of alpha helices
  • Made of 2 chains, almost entirely of beta sheets

Question 17

Question
2 Alpha helices dock Alt. Ox in the membrane
Answer
  • True
  • False

Question 18

Question
How does the substrate get to Alt. Ox's active site?
Answer
  • Through a hydrophobic cavity
  • It is 'caught' by alpha helices extending into the cytoplasm
  • It uses membrane phospholipids which move through it

Question 19

Question
The following shows the core of Alt. Oxidase- what's contained in it?
Image:
Alt._Ox._Core (image/png)
Answer
  • 2 Fe atoms
  • 2 Cu atoms
  • Linked by hydroxyl groups
  • Linked by amide groups
  • From 4 leucine residues
  • From 4 glutamine residues
  • And 2 Histadine &1 Tyrosine residues

Question 20

Question
The Histadine/Tyrosine residues can be used to inhibit Alt. Ox
Answer
  • True
  • False

Question 21

Question
To travel through the channel and reach the active site, inhibitors must be...
Answer
  • Hydrophobic
  • Hydrophillic

Question 22

Question
Different co-ordinations of the aa in the Alt. Ox core can alter the function of it
Answer
  • True
  • False

Question 23

Question
Alt. Ox. is a terminal respiratory chain oxidase
Answer
  • True
  • False

Question 24

Question
Where is Alt. Ox found, in infected humans?
Answer
  • Between Complexes 1 & 2
  • Between complexes 2 & 3
  • Between complexes 3 & 4

Question 25

Question
Alternative Ox is used as a stress protein in pathogens, and an oxygen scavenger in plants
Answer
  • True
  • False

Question 26

Question
Which of the following use Alt. Ox?
Answer
  • American/Asian Skunk Cabbage
  • Dead Horse Arum
  • Sacred Lotus
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