Copy and Edit

Mutagenesis

Description

Year 2 Quiz on Mutagenesis, created by gina_evans0312 on 29/12/2013.
gina_evans0312
Quiz by gina_evans0312, updated more than 1 year ago
gina_evans0312
Created by gina_evans0312 almost 11 years ago
936
0
0

Resource summary

Question 1

Question
What is site directed mutation?
Answer
  • Where mutagenesis is directed towards a specific site
  • Where certain sites are more prone to mutagenesis than others
  • Where certain sites cannot be mutated and must be worked around

Question 2

Question
What form must the DNA be in for Site Directed Mutagenesis?
Answer
  • ssRNA
  • ssDNA
  • dsDNA

Question 3

Question
The primer for site directed mutagenesis is designed to have one or more mismatches
Answer
  • True
  • False

Question 4

Question
Once the mismatch has been achieved, how is it used?
Image:
Site_Directed (image/png)
Answer
  • During repair, the old base will be replaced with a new match
  • During replication, half the daughter cells will carry the mutation

Question 5

Question
What is specific about the DNA used in the Kunkel Method?
Answer
  • It's dsDNA
  • It's ssDNA
  • From the Slo + form of E.Coli
  • From the dut ung. form of E.Coli

Question 6

Question
What is Incorporated into the original DNA in order to make it susceptible to degrading
Answer
  • Uracil
  • Methyl-cytosine
  • Thymidine-3-phosphate

Question 7

Question
Like with site directed, the primer annealed to the DNA is designed to create mismatches
Answer
  • True
  • False

Question 8

Question
In both site directed and the Kunkel method, how is the primer extended?
Answer
  • DNA polymerase
  • Taq Polymerase
  • RNA polymerase

Question 9

Question
Once the DNA has been inserted, what happens to the original DNA strand?
Answer
  • The Uracil cause it to be partially degraded
  • The Methyl-cytosine cause it to be partially degraded
  • The Thymidine-3-Phosphate cause it to be partially degraded
  • Being removed alone with the bp next to them

Question 10

Question
The partially degraded strand is them re-synthesised by DNA repair proteins, finalising the mutation
Answer
  • True
  • False

Question 11

Question
Which method has a way of determining which plasmid has the mutation?
Answer
  • Site- Directed
  • Altered Site
  • Kunkel Method

Question 12

Question
What is particular about the plasmid used in the Altered Sites method?
Answer
  • It contains a non-functional Amp resistance gene
  • It contains a gene for DNA repair proteins
  • It's dsRNA

Question 13

Question
What oligonucleotide primers are used during Altered Site mutagenisis?
Answer
  • Mutant primer
  • Ampicillin 'Repair' primer
  • Ampicillin Non-Function primer

Question 14

Question
What is the result, after DNA replication has occurred?
Answer
  • The desired plasmid has the mutation, and tetracyline and ampicillin resistance
  • The desired plasmid has the mutation and tet resistance but no ampicillin resistance
  • The desired plasmid has the mutation and ampicillin resistance but no tetracyline resistance

Question 15

Question
What causes the mutagenesis in the 'Quickchange' PCR method?
Answer
  • A two primers containing the mutation
  • Rapid replication of the Site Directed mutant
  • One primer contains a mutation, the other is used to create the mutated plasmid
  • The original DNA is then degraded, leaving only the mutated plasmid

Question 16

Question
Why is Dpn used to degrade the original DNA?
Answer
  • It degrades only methylated DNA
  • It degrades only acetylated DNA
  • It degrades only ibiquitinated DNA

Question 17

Question
Site directed mutagenesis is unique in that no structural knowledge of the protein is required
Answer
  • True
  • False

Question 18

Question
State the two methods by which non-directed mutation occurs in evolution
Answer
  • Gradual accumulation of beneficiary mutations
  • Gradual loss of unnecessary DNA
  • Recombination of genes to form hybrid protein

Question 19

Question
These random mutations can be mimicked in a lab
Answer
  • True
  • False

Question 20

Question
Random mutagenesis works best by introducing many mutations at the same time
Answer
  • True
  • False

Question 21

Question
What will happen when this DNA replicates?
Image:
Degenerate_Primer (image/png)
Answer
  • The amino acid marked by the N's will be randomised
  • The Leu will be deleted
  • The N's are a stop codon, halting the gene

Question 22

Question
Primers can be more specific and limit the choice of potential aa further
Answer
  • True
  • False

Question 23

Question
Name an Error prone DNA polymerase
Answer
  • Taq
  • T7
  • Pol

Question 24

Question
Name the two conditions necessary for Error Prone mutagensis
Answer
  • An error prone polymerase
  • Conditions designed to increase error
  • No DNA repair enzymes
  • Restriction enzymes

Question 25

Question
DNA shuffling is used to create fusion proteins from two unrelated genes
Answer
  • True
  • False

Question 26

Question
Why does gene shuffling work?
Answer
  • Ligation repairs DNA errors
  • Related genes have overlapping areas
  • Related genes can ligate together with less error

Question 27

Question
What protein is used to randomly cut up two related genes for DNA shuffling?
Answer
  • DNAase I
  • DNAase III
  • EcoRV
  • EcoRI

Question 28

Question
The randomly cut up sequences are then ligated back together
Answer
  • True
  • False

Question 29

Question
When creating hybrids, the traditional approach of screening colonies for hybrids is not efficient- so we use robots
Answer
  • True
  • False

Question 30

Question
Name the two main methods of analysing mutants quickly
Answer
  • Surface display of proteins
  • Elution
  • Chemical compartmentalisation

Question 31

Question
The advantage of Protein Surface Display in improved mutations is?
Answer
  • Improved mutations will bind more strongly to immobalised substrate
  • They react more strongly to dyes
  • They don't degrade as easily

Question 32

Question
Once the variants are bound, they are washed so strongly only powerfully binding variants remain bound
Answer
  • True
  • False

Question 33

Question
Once the most improved variation has been identified, what can happen to it?
Answer
  • It's washed off and sequenced
  • It's washed off and improved again

Question 34

Question
Chemical Compartmentalisation uses an oil/acetyl emulsion
Answer
  • True
  • False

Question 35

Question
Why are the droplets important?
Answer
  • They make reading the DNA easier
  • Each droplet contains an individual mutation
  • Each droplet contains mutants that compliment each other

Question 36

Question
The DNA cannot be transcribed or translated in the droplets, only sequenced
Answer
  • True
  • False

Question 37

Question
Once the protein has been made, how are they assayed?
Answer
  • Robots put them on multi-well dishes for assay
  • Fluorescent activated cell sorters are used

Question 38

Question
How is fluorescence used for separation in Fluorescent Activated Cell Sorters?
Answer
  • The more fluorescent product is produced, the better the enzyme
  • The stronger the enzyme-substrate binding, the better the enzyme
  • The more fluorescent substrate is used, the better the enzyme
Show full summary Hide full summary

Want to create your own Quizzes for free with GoConqr? Learn more.

Similar

PCR
gina_evans0312
DNA Fragmentation and Cloning Vectors
gina_evans0312
Extract DNA
gina_evans0312
Creating an E.Coli Library
gina_evans0312
Clone Identification and Expression
gina_evans0312
Head, Neck and Back 2nd Sem- Anatomy 2nd Year- PMU
Med Student
Respiratory System 2nd Year PMU Anatomy
Med Student
Respiratory System Year 2/
Sole C
Fiction or NonFiction Year 1
Judy Stephenson
Fiction or NonFiction Year 2
Judy Stephenson
Anatomy Year 2 - Head, Neck and Back
Sole C
Browse Library