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Clone Identification and Expression

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Year 2 Quiz on Clone Identification and Expression, created by gina_evans0312 on 29/12/2013.
gina_evans0312
Quiz by gina_evans0312, updated more than 1 year ago
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Created by gina_evans0312 over 10 years ago
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Resource summary

Question 1

Question
A radioactive probe can take what forms?
Answer
  • ssDNA
  • RNA
  • Denatured dsDNA
  • Oligonucleotides

Question 2

Question
Probes can be
Answer
  • Radioactive
  • Fluorescent
  • Use Ig's

Question 3

Question
Name the two ways probes can be designed
Answer
  • Guesswork
  • Codon Bias

Question 4

Question
What is codon bias?
Answer
  • Where cells prefer one codon of an aa to another
  • Where cells will reject certain codons due to what they contain
  • Where some cells can only make tRNA's for certain codons

Question 5

Question
80% of Phe codons are TTC, 20% are TTT
Answer
  • True
  • False

Question 6

Question
If the protein is an enzyme it can be found by adding the substrate
Answer
  • True
  • False

Question 7

Question
State the possible forms of Ig's for protein separation?
Answer
  • Immobalised on a column
  • Removable from solution

Question 8

Question
Probes needn't be 100% accurate to work
Answer
  • True
  • False

Question 9

Question
For a Western Blot, how is the DNA stuck to the membrane?
Answer
  • Denatured DNA sticks to membrane
  • Bacteria are lysed to release DNA
  • DNA washes off

Question 10

Question
Once the DNA has been bound, what happens next?
Answer
  • A probe is added
  • The membrane is run through a gel
  • The membrane is stained

Question 11

Question
In an expression vector, where is the new gene added?
Answer
  • The Multiple Cloning Site
  • The Promotor Region
  • Next to the origin

Question 12

Question
Name the parts on the vector
Image:
Expression_Vector (image/png)
Answer
  • Origin
  • Antibiotic Resistance
  • Promoter
  • Terminator
  • Multiple Cloning Site

Question 13

Question
More compliated vectors have proteins tagged for purification
Answer
  • True
  • False

Question 14

Question
What is involved in His Chain Purification?
Answer
  • The addition of a His chain to the N terminus
  • The removal of a His chain from the protein
  • The use of a His chain to cut unwatned proteins

Question 15

Question
His Purification uses Histadine's affinity for what metal?
Answer
  • Ni2+
  • Fe2+
  • Na+

Question 16

Question
His-tagged proteins will bind to Nickel in a column. Others that don't bind will be washed off.
Answer
  • True
  • False

Question 17

Question
The PET system involves using a promoter for the protein that you can control the expression of
Answer
  • True
  • False

Question 18

Question
What promoter is used in the PET system to control protein expression?
Answer
  • T7
  • T8
  • T9

Question 19

Question
In the PET system, what promoter is used to control the T_ expression?
Answer
  • Lac promoter
  • Slo Promoter
  • Pap promoter

Question 20

Question
What is required for a good shuttle vector?
Answer
  • Replicable in both hosts
  • Selectable in both hosts
  • Expressable in both hosts

Question 21

Question
Shuttle vectors are usually plasmids with additional sequences
Answer
  • True
  • False

Question 22

Question
Shuttle vectors for yeast/fungi need
Answer
  • Special origin
  • Antibiotic/metabolic marker

Question 23

Question
A baclovirus would be used as a vector into what?
Answer
  • Insects
  • Plants
  • Mammals

Question 24

Question
Secretion of certain proteins can be caused by what?
Answer
  • Disulphide bonds
  • Acetylation
  • Methylation

Question 25

Question
Why are there two origins in the Yeast shuttle vector?
Image:
Shuttle_Vector (image/png)
Answer
  • One for the yeast to use
  • One for the bacteria to use

Question 26

Question
What is the method of selection in yeast?
Image:
Shuttle_Vector (image/png)
Answer
  • URA3- allows growth in the absence of Uracil
  • TRA3- allows growth in the absence of thymidine
  • GRA3- allows growth in the absence of guanine

Question 27

Question
Like bacteria, yeast cannot take up linear DNA
Answer
  • True
  • False

Question 28

Question
What does a YAC contain that a plasmid does not?
Answer
  • Non-useful genes
  • Yeast centromere
  • Yeast telomeres

Question 29

Question
Yeast can allow DNA to be directly inserted into its genome
Answer
  • True
  • False

Question 30

Question
How can yeast accept DNA into its genome?
Answer
  • Homologous recombination
  • Non-homologous end joining
  • Direct injection

Question 31

Question
In order to directly insert a gene, you must flank the gene with identical stretches of the part of the genome you want to insert it in
Answer
  • True
  • False

Question 32

Question
Plants would use an aglobacterium T1 plasmid
Answer
  • True
  • False

Question 33

Question
How do you replicate transformed plant cells?
Answer
  • Grown them as plantlets
  • Clone them
  • Asexually reproduce them

Question 34

Question
Mammal shuttle vectors are the same as yeast vectors, but the yeast origin is replaced with what?
Answer
  • An SV40 promoter
  • A UG35 promoter
  • A FT12 promoter

Question 35

Question
Retroviruses can also be used to insert transgenes into mammilian genomes by packing the transgene in a retroviral capsule
Answer
  • True
  • False
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