456291
Description
Quiz by gina_evans0312, updated more than 1 year ago
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Created by gina_evans0312
over 10 years ago
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Question 1
Question
Gene synthesis can be fully automated cheaply
Answer
- True
- False
Question 2
Question
Why is genome synthesis difficult?
Answer
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Chemical synthesis is limited to 100nt at a time
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Manipulation of large fragments is difficult
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Vectors are limited
Question 3
Question
Synthesis of a genome must be done in small, overlapping parts
Answer
- True
- False
Question 4
Question
How many kbpairs can PCR be used for?
Answer
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1-5
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5-10
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10-15
Question 5
Question
What is essential when creating small fragments to be joined together later in clones?
Answer
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The fragments must overlap
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The fragments MUST be 7kb long
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The fragments cannot contain introns
Question 6
Question
When transplacting the synthetic genome between clones, what must not be present?
Answer
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Proteins
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DNA
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RNA
Question 7
Question
How is the receptor organism for the synthetic genome made able to receive it?
Answer
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The cell wall is treated with chemicals to make it permeable
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The DNA is directly microinjected into the cell
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The cell absorbs the DNA with other nutrients
Question 8
Question
Once the synthetic genome has been added to the cell, it divides as it contains two genomes
Answer
- True
- False
Question 9
Question
Why synthetic organisms?
Answer
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To see if we can
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To improve/redesign regulatory networks
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To produce protective genes
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Biofuels/biopharmecuticals
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