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DNA (labeling) for biochem and cell biology (lecture 2)

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1st year Biochemistry and molecular biology Quiz on DNA (labeling) for biochem and cell biology (lecture 2), created by MrSujg on 29/11/2015.
MrSujg
Quiz by MrSujg, updated more than 1 year ago
MrSujg
Created by MrSujg about 9 years ago
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Resource summary

Question 1

Question
Label the image
Image:
97ba91f2-c354-451e-8038-fa8309d5ae4e (image/png)
Answer
  • 1'
  • 2'
  • 3'
  • 4'
  • 5'
  • alpha
  • Beta
  • gamma
  • base

Question 2

Question
What base is it ?
Image:
eaa093c2-a229-4330-ac20-3c1be45d3eb7 (image/png)
Answer
  • Adenine
  • Guanine
  • Cytosine
  • Thymine
  • Uracil

Question 3

Question
What base is it ?
Image:
4f7dbed1-80e3-4bcd-8896-0e7cc9065793 (image/png)
Answer
  • Guanine
  • Adenine
  • Cytosine
  • Thymine
  • Uracil

Question 4

Question
What base is it?
Image:
694ddb2d-0a6e-45fe-906f-f8558ee47aba (image/png)
Answer
  • Cytosine
  • Adenine
  • Guanine
  • Thymine
  • Uracil

Question 5

Question
What base is it?
Image:
a2296de7-40b2-4362-ab3f-98d6e43bed87 (image/png)
Answer
  • Thymine
  • Adenine
  • Uracil
  • Cytosine
  • Guanine

Question 6

Question
What base is it?
Image:
1da0c00c-fc24-42a5-8fa8-da2d5380d6b0 (image/png)
Answer
  • Uracil
  • Adenine
  • Guanine
  • Tymine
  • Cytosine

Question 7

Question
Identify primers
Image:
9d559227-f204-493c-96c5-c50babb61ca7 (image/png)
Answer
  • 3'
  • 5'

Question 8

Question
Label the image
Image:
6da2753f-30da-4d2c-a57c-539e77b339bf (image/png)
Answer
  • 5'
  • 3'
  • 5'
  • 3'
  • alpha
  • beta
  • gamma
  • O
  • C
  • S
  • H
  • N
  • F
  • DNA polymerase I
  • DNA polymerase II
  • DNA polymerase III

Question 9

Question
Label the image
Image:
32cffeda-7ef1-4b04-a973-2837895aebaf (image/png)
Answer
  • Minor groove
  • Major groove

Question 10

Question
Label the image
Image:
a142803c-4ca0-43b2-bbe1-3a6141b89c0f (image/png)
Answer
  • Guanine
  • Cytosine
  • Thymine
  • Adenine

Question 11

Question
Determine the primers
Image:
b1f5e341-d54a-4dd3-9f52-ba75d4dbfee8 (image/png)
Answer
  • 5'
  • 3'
  • 3'
  • 5'
  • 3'
  • 5'
  • 5'
  • 3'

Question 12

Question
Label the image
Image:
aa762e2c-7cbe-4134-82bc-6577de8f9f51 (image/png)
Answer
  • A-DNA
  • B-DNA
  • Z-DNA

Question 13

Question
Label the image
Image:
3898de21-03d4-46d8-80c0-277b8e37b6f7 (image/png)
Answer
  • Conservative mechanisms
  • Semiconservative mechanism

Question 14

Question
Label the image
Image:
98aa9ee9-9028-456d-8c43-fc2bf06f14f5 (image/png)
Answer
  • Origin
  • Growing fork

Question 15

Question
Label the image
Image:
354ecee5-baa7-461d-a1ef-374333359b3a (image/png)
Answer
  • EcorRI restriction site
  • Origin
  • Replication bubble
  • Time of replication

Question 16

Question
Labelt the replication cartoon
Image:
c7a69787-e289-42b7-80ed-2801a3fa509c (image/png)
Answer
  • Primase
  • SIngle0stranded region of parent DNA
  • RNA primer
  • DNA polymerase III
  • Newly made DNA
  • DNA polymerase I
  • RNA still to be replaced
  • Newest DNA
  • Completed daughter cell

Question 17

Question
Label the cartoon showing the unwinding at the origin
Image:
f63fc1cf-bad8-47b6-a967-a46d3921a33c (image/png)
Answer
  • 9-mers
  • Negatively supercoiled template
  • DnaA
  • ATP
  • Initial complex
  • Open complex
  • DnaC
  • DnaB (helicase)
  • Prepriming complex

Question 18

Question
Label the cartoon showing Simultaneous synthesis on both strands
Image:
9ef4a3c0-4be0-419c-823c-5b4b55e09987 (image/png)
Answer
  • Point of joining
  • Lagging strand
  • Okazaki fragment
  • Short RNA primer
  • Leading strand
  • Parental DNA duplex
  • Daughter duplex
  • Direction of fork movement

Question 19

Question
Label the cartoon showing the participitation of topoisomers in DNA unwinding
Image:
a577fc9f-ba3c-4bac-9498-9daff7642b2b (image/png)
Answer
  • Covalently closed circular template
  • DNA replication
  • Unwound parental duplex
  • Negative supercoils
  • Positive supercoils
  • Overwound region

Question 20

Question
Label DNA polymerase
Image:
35a86cb2-ac0d-4e63-9240-5612df9d0131 (image/png)
Answer
  • Fingers
  • Thumb
  • Growing strand
  • Template strand
  • Exo
  • Palm
  • Pol

Question 21

Question
Label the image showing mismatch repair
Image:
c4c569cb-23b4-438f-a8ae-961efeca3a5f (image/png)
Answer
  • Daughter strand
  • Parental strand
  • Mismatched base pair
  • Mismatch recognition
  • MutS
  • MutH
  • MutH cleavage
  • Gap repair by DNA polymerase and ligase

Question 22

Question
Label the cartoon showing excision repair
Image:
5fb753e5-d05a-476d-bb81-942d4ad07d81 (image/png)
Answer
  • Initial damage recognition
  • 23B
  • XP-C
  • Opening of DNA double helix
  • TFIIH
  • XP-G
  • RPA
  • XP-F and XP-G endonucleases
  • DNA polymerase and DNA ligase

Question 23

Question
Label the image showing PCR
Image:
29516573-140e-440c-b28f-033e27392edf (image/png)
Answer
  • DNA Denaturation, annealing of primers
  • Elongation of primers
  • DNA denaturation; annealing or primers 2
  • Elongation of primers 2

Question 24

Question
Label the image
Image:
7889a418-6e28-4396-acae-ecd035ba5303 (image/png)
Answer
  • RNA polymerase I
  • RNA polymerase II
  • RNA polymerase 3

Question 25

Question
Label the image showing the Transcription in Prokaryotes
Image:
008be8d7-b8c0-4757-8426-80cd106a4f30 (image/png)
Answer
  • Promoter DNA
  • -35 sequence
  • 10 sequence
  • Coding strand
  • Template strand
  • Startpoint

Question 26

Question
Labelt the image showing A-Binding Domains (DBDs)
Image:
6ce4192a-816c-4de6-83dd-d1e7029bcbc3 (image/png)
Answer
  • Helix-turn-helix motif
  • The lambda repressor
  • Zinc finger motif
  • Leucine zipper motif
  • Helix-loop-helix motif

Question 27

Question
Label the image showing the lac operon activation
Image:
a17d1ab7-041c-4644-b4cc-2a54a72b78d2 (image/png)
Answer
  • Beta-galactosidase
  • RNA polymerase bound to promoter
  • lac-mRNA
  • Lactose
  • RNA polymerase transcribing the operon
  • lacI gene
  • Inactive form of repressor

Question 28

Question
Label the image showing the regulation of the lac Operon
Image:
c5c2f9ba-30ba-4412-89bd-33b192cd65e6 (image/png)
Answer
  • Promoter
  • CAP site
  • CAP
  • a70-Pol
  • lac repressor
  • Lactose
  • cAMP

Question 29

Question
Label the image showing Eukaryotic promoters
Image:
3e346027-d6c6-4e0c-9b13-28fb2881070c (image/png)
Answer
  • GC box
  • CAAT box
  • TATA box
  • Proximal control elements
  • Core promoter
  • Start of transcription
  • Poly (A) site
  • 5' untranslated region (leader)
  • 3' untranslated region (trailer)

Question 30

Question
label the image showing General Eukaryotic Transcription Factors
Image:
4d83b5f1-bcbd-4cfe-946f-73ad4570814b (image/png)
Answer
  • Core promoter
  • Startpoint
  • TFIID
  • TFIIA
  • TFIIB
  • TFIIF
  • RNA polymerase 2
  • TFIIE
  • TFIIH
  • Transcription begins

Question 31

Question
Label the image showing Additional Eukaryotic Transcription Factors
Image:
0ddef686-0faa-4bfd-a357-fa0cfb066fe2 (image/png)
Answer
  • Enhancer
  • Core promotor
  • TATA
  • Inr
  • Gene
  • Activators
  • Coactivators
  • Enhanceosome
  • TFIID
  • Activator and Coactivator
  • Transcription

Question 32

Question
label the image showing Tissue-Specific Gene Expression
Image:
83151982-9958-4315-afd5-6c724231c60c (image/png)
Answer
  • Control elements
  • Core promoter
  • Regulatory transcription factors
  • General transcription factors
  • RNA polymerase
  • Transcription complex
  • DNA
  • RNA transcript

Question 33

Question
Label the image showing how the final mRNA is made
Image:
c354a8e3-273d-416f-8c0c-774c4679b0d0 (image/png)
Answer
  • Exon
  • Intron
  • Poly (A) site
  • Termination sites
  • Transcription 5' capping
  • Cleavage at poly (A) site
  • Polyadenylation
  • RNA splicing

Question 34

Question
Labe the image showing Microarrays: Genome-Wide Analysis
Image:
4c14d5c9-3afe-4542-bb21-0025b83b3070 (image/png)
Answer
  • RNA isolation
  • Reverse transcriptase labeling
  • Combine targets
  • Hybridaze to microarray

Question 35

Question
Label the image showing the molecular Dogma
Image:
3d5f7294-4159-45cc-9661-7116bc7deb07 (image/png)
Answer
  • Transcription
  • Translation
  • Assembly
  • tRNA
  • rRNA
  • Ribosomes
  • Polypeptides
  • Proteins
  • Ribosomal proteins

Question 36

Question
Label the image
Image:
188e25d1-ef20-4a80-a195-dab16bcb2a5c (image/png)
Answer
  • Primary RNA transcript
  • 5'
  • 3'
  • DNA
  • Promoter
  • Transcription
  • Coding sequence
  • Upstream
  • Downstream

Question 37

Question
Follow the genetic code to match the appropriate amino acid to each codon
Image:
9f0c4d2f-c570-4736-8759-460cdfacd987 (image/png)
Answer
  • Ala
  • Cys
  • Leu
  • Arg
  • Ile
  • Val
  • Tyr
  • Glu

Question 38

Question
Label the type of the mutation
Image:
aad2b58a-2125-4ddb-8f29-d7fa6f906d09 (image/png)
Answer
  • Frameshift
  • Missense
  • Nonsense

Question 39

Question
Identify the type of ribosome, and the size of the assembled unit
Image:
b441155e-7692-40b4-af8a-d2d1d07be6fd (image/png)
Answer
  • Prokaryotic
  • Eukaryotic (vertebrate)
  • 70S
  • 80S

Question 40

Question
Label the image showing aminoacyl-tRNAsynthetases
Image:
baa4b803-cf3e-4ad9-9e39-48755e355485 (image/png)
Answer
  • High-energy ester bond
  • Amino acid (Phe)
  • mRNA
  • Aminoacyl-tRNA
  • tRNA specific for Phe

Question 41

Question
Label the image
Image:
080fb1c4-1028-4c23-ba9c-9f2f04bdeeb3 (image/png)
Answer
  • Adenosine deaminase
  • Adenosine
  • Inosine

Question 42

Question
Label the initiator methionine
Image:
3d29c23b-2174-46e0-b136-635273233ac0 (image/png)
Answer
  • Formyl group
  • Carboxyl group
  • Acetyl group
  • Phosphate group

Question 43

Question
Label each mRNA
Image:
bcc16fb4-5a72-4b5d-be77-3183db133ab6 (image/png)
Answer
  • Prokaryotic mRNA
  • Eukaryotic mRNA

Question 44

Question
Label the ribosome sites
Image:
a6e21ea5-1b72-4ce0-8d08-b98dfdcec962 (image/png)
Answer
  • A site
  • P site
  • E-site
  • mRNA-binding site
  • mRNA
  • Growing peptide chain

Question 45

Question
Label the image showing translation
Image:
5b815481-de9a-46a3-a414-fbdc881e5324 (image/png)
Answer
  • P site
  • E site
  • A site
  • Release factor
  • free tRNA
  • Polypeptide
  • Ribosomal subunits
  • mRNA

Question 46

Question
Label the image showing translation mechanism
Image:
b6e85445-9c31-41bd-9cdd-7706e8013886 (image/png)
Answer
  • AUG start codon
  • UAG stop codon
  • mRNA
  • Initiation
  • Elongation
  • Termination
  • Release factor
  • Completed polypeptide
  • Recycling of translational components
  • Ribosomal subunits

Question 47

Question
Label the image showing western blotting
Image:
4753de13-785f-4e6c-bd4e-468584298cc8 (image/png)
Answer
  • Electrophoresis and transfer
  • Antibody detection
  • Chromogenic detection
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