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DISAGGREGATION OF EXPLANT

Description

Flowchart on DISAGGREGATION OF EXPLANT, created by Aaff rin on 03/01/2018.

Flowchart by Aaff rin, updated more than 1 year ago

	Created by Aaff rin about 7 years ago

Resource summary

Flowchart nodes

DISAGGREGATION OF EXPLANTS

FINE DISSECTION

MECHANICAL DISAGGREGATION

COLD TRYPSIN

WARM TRYPSIN

COLLAGENASE

DISPERSED PRIMARY CULTUTURE

SUBCULTURE

PRIMARY EXPLANT

TRANSFER

2ND EXPLANT

OVER NIGHT INCUBATION

LONG INCUBATION

CENTRIFUGE

CELL LINE

OUTGROWTH

RESUSPEND AND SEED

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