DISAGGREGATION OF EXPLANT

Description

Flowchart on DISAGGREGATION OF EXPLANT, created by Aaff rin on 03/01/2018.
Aaff rin
Flowchart by Aaff rin, updated more than 1 year ago
Aaff rin
Created by Aaff rin almost 7 years ago
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Resource summary

Flowchart nodes

  • DISAGGREGATION OF EXPLANTS
  • FINE DISSECTION
  • MECHANICAL DISAGGREGATION
  • COLD TRYPSIN
  • WARM TRYPSIN
  • COLLAGENASE
  • DISPERSED PRIMARY CULTUTURE
  • SUBCULTURE
  • PRIMARY EXPLANT
  • TRANSFER
  • 2ND EXPLANT
  • OVER NIGHT INCUBATION
  • LONG INCUBATION
  • CENTRIFUGE
  • CELL LINE
  • OUTGROWTH
  • RESUSPEND  AND SEED
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