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Description
Flashcards by gina_evans0312, updated more than 1 year ago
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Created by gina_evans0312
almost 11 years ago
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| Question | Answer |
| Marker | Loci/Mendelian characteristic that can be traced through a pedigree |
| Role of a marker | Act as a reference point in linkage studies |
| SNP | Small Nucleotide Polymorphism- small code variations between individuals |
| Polymorphism | Many alleles |
| More polymorphic = | More useful, as individuals are more likely to be heterozygotes |
| If recombinants between an SNP & a gene are uncommon... | The two are mapped close together |
| RFLP | Restriction Fragment Length Polymorphism |
| RFLP relies on... | One allele having a Polymorphic Restriction Site |
| DNA probe | Done to find Polymorphic Restriction Site |
| Restriction Enzyme | Used to cut at Polymorphic Restriction Site to create multiple pieces |
| Detection of RFLP | Run a Southern Blot or Gel Electrophoresis for genetic material |
| Homozygosity in RFLP | 1 line on Southern Blot/Gel |
| SSLP/Microsatellites | Single Sequence Length Polymorphism |
| Detecting Microsatellites/SSLP's | PCR amplification using markers long around M.S |
| Microsatellite Primers | Fluorescent, 1-4bp long |
| Using Gel Eletrophoresis | Use PCR before cutting to ensure enough material |
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Image:
SSLP (image/png)
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SSLP/Microsatellites |
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Image:
SSLP_Analysis (image/png)
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A is homozygous, B is heterozygous |
| SNP | Single Nucleotide Polymorphism- areas that differ by a single base, even in highly conserved areas |
| Oligonucleotide Hybridisation | Used for detecting SNP's- very specific |
| Detecting SNP's- Fluorescent Tag | Released when oligonucleotide probe binds to the DNA |
| Detecting SNP's- Quencher Tag | Prevents Fluorescent tag from Fluorescing unless hybrid has been formed |
| Detecting SNP's- Hybrids | Once oligonucleotide has bound, base pairing determines whether binding is stable or unstable |
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