327207
Description
Flashcards by gina_evans0312, updated more than 1 year ago
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Created by gina_evans0312
over 10 years ago
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| Question | Answer |
| While inactive, tyrosine kinase exists as | A monomer |
| The binding of the ligand causes the ....?... of tyrosine kinase | Dimerisation |
| The structural change of tyrosine kinase causes | Partial activation |
| What allows tyrosin kinase to become fully active? | The phosphorylation of the activation lip |
| When the protein is fully activation, which protein is phosphorylated? | Tyrosine kinase |
| This phosphorylation provides a docking site for which protein? | GRB2 |
| What does the activation of RAS require? | A complex of protein linked to tyrosine kinase |
| Which part of GRB2 binds to GEF? | SH3 |
| The SH2 subunit of GRB2 binds where? | To the phosphorylated tyrosine kinase |
| Why doesn't GRB2 bind to GEF all the time? | Because only binding to phoshorylated T.K exposes the SH3 domain |
| Adaptor Protein | Allows 2 unlike proteins to bind |
| What aa-rich domain does the SH3 domain of GRB2 bind to? | The proline rich region of GEF |
| Where is inactive RAS found in a cell? | In the membrane bound to GDP |
| What does GEF stand for? | Guanine nucleotide exchange factor |
| Why does GEF binding activate RAS? | It replaces GDP with GTP, activation it |
| What is the main function of active RAS? | Downstream cascade causing cell division |
| How is RAS deactivated? | GAP- GTPase activating protein causes GTP hydrolysis |
| How long is RAS active for (in general) | One minute |
| How does GEF activate RAS? | Inserts an alpha helix to open the switches, so GDP is replaced by GTP |
| Name the two aa that hold the RAS switch closed | Gly60 & Thr 35 |
| What is the first protein RAS activates? | RAF |
| 14-3-3 protein | Binds to RAF and holds it in a folded state |
| Phosphorylation of what aa holds RAF inactive? | Serine |
| The binding of RAS to RAF causes what to be displaced? | 14-3-3 protein |
| RAS binding causes what to be phosphorylated? | Kinase domain |
| RAS binding causes what to be dephosphorylated? | The N terminus |
| Once RAF has been unfolded, it phosphorylates | MEK |
| MAPK is phosphorylated by | MEK |
| MEK is unusual because | It can phosphorylate Ser, Thr & Tyr |
| Function of MPK | Phosphorylation of nuclear transcription factors |
| How is MAPK activated? | Phosphorylation of the activation lip |
| Name the P protein activated by MAPK | p90-rsk |
| p90rsk activates | PIPG |
| MEK stands for... | MAP Kinase Erk Kinase |
| MEK stands for | MAPK Erk Kianse |
| How does MEK phosphorylated MAPK? | Tyrosine residue on activation lip, then the newly exposed Threonine residue |
| p90rsk is sensitive to... | Insulin |
| PP1G stands for | Protein phosphatase-1-G |
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