Biotech I

Question	Answer
Acetobacter	Acetic acid Obligate aerobes
Vitamin C	Acetobacter incomplete [O]
AB	Streptomyces
Swiss cheese	propionic acid bacteria
Insulin efficiency	Small peptide Easier to construst aGene than making from larger precursor protein
Codon bias	Codon usage preference varies btw. org Many need to alter codons used to fit w/ those recognised by your bacterial species
Other problems w/ mammalian genes in bacteria	Protein may require post-translational mod May be degraded by host May be toxic to host
Production of metabolites requires	Single gene won't suffice Build up whole metabolic pathway
Pathway engineering	Assemble met. pathway using genes from 1 or more org. to produce large amounts of metabolite
Indigo production (4)	tryptophan tryptophanase in E.coli Indole Napthalene oxygenase from pseudomonas
Biomediation	Org. to clean up enviro pollutants
Microbial plastic	PHAs
Microbial leaching	Low grade ore crushed + dumped in pattern w/ ^ SA Fe3+ crucial [RA]
Metagenomes	Collective genomes of all organisms growing in an environment
Gene mining	Isolate potentially useful novel genes w/o having to culture the org.
Targeted gene mining	Metagenomics used to screen directly for Ez. w/ certain properties.

Resource summary

	Created by J yadonknow almost 6 years ago