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Protein-DNA Interactions- DNA Repair

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Structural Basis for Biological Function (DNA-Protein Interactions) Quiz on Protein-DNA Interactions- DNA Repair, created by gina_evans0312 on 24/12/2013.
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Quiz by gina_evans0312, updated more than 1 year ago
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Created by gina_evans0312 over 10 years ago
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Question 1

Question
Which of the following are caused by hydrolysis?
Answer
  • Nicks
  • Abasic Sites
  • Cross Links
  • Ds Breaks

Question 2

Question
Which of the following are caused by Radical Oxygen Species?
Answer
  • Bulky alkylation damage
  • Abasic sites
  • ds breas
  • ss breaks
  • Nicks

Question 3

Question
What causes Alkylation Damage?
Answer
  • S-adenosyl methionine
  • Radical oxygen species
  • Interacts with 6th O on guanine
  • Interacts with 3rd O on guanine

Question 4

Question
Methylation of guanine results in unstable base pairing
Answer
  • True
  • False

Question 5

Question
Over two cycles of replication, a methylated C-G will be replaced with an?
Answer
  • A-T
  • C-C
  • G-T

Question 6

Question
What protein catalyses Direct Reversal of Guanine methylation?
Answer
  • Alkyl-Guanine Transferase
  • Adenosyl-Guanine Transferase
  • Adenyl-Guanine Transferase

Question 7

Question
The enzyme involved in direct reversal catalyses the transfer of CH3 to the enzyme, killing it
Answer
  • True
  • False

Question 8

Question
How does the Direct Reversal enzyme 'flip out' the damaged guanine?
Answer
  • A helix-helix motif
  • A helix-turn-helix motif
  • A sheet-turn-sheet motif

Question 9

Question
The enzyme for direct reversal is not specific and so works globally
Answer
  • True
  • False

Question 10

Question
What is the reading head of the Direct Reversal enzyme made of?
Answer
  • Ala & Gly
  • Ser & Val
  • Tyr & Ser

Question 11

Question
The enzyme binds to the major groove using an HTH motif
Answer
  • True
  • False

Question 12

Question
Which happens first in the CH3 transfer?
Answer
  • Lys pushes H onto Tyr
  • Lys pushes CH3 onto Tyr
  • Tyr pushes H onto Lys

Question 13

Question
The new H+ pushes e-....
Answer
  • Onto CH3
  • Onto O bound to CH3
  • Onto Lys bound to CH3

Question 14

Question
The e- added to the O pushes the CH3 onto the Lysine
Answer
  • True
  • False

Question 15

Question
Which of the following recognises the widowed G and fills the gap?
Answer
  • Arg on the head of the reading head
  • Lys on the head of the reading head
  • Val on the head of the reading head

Question 16

Question
UV irradiation of _ causes _?
Answer
  • Consecutive pyrimidines
  • Consecutive purines
  • Opens a 5-6 bond
  • Opens a 3-4 bond
  • Opens a 4-6 bond

Question 17

Question
A phosphobutane photo-dimer is formed
Answer
  • True
  • False

Question 18

Question
Why is UV radiation dangerous?
Answer
  • It alters DNA conformation
  • So helicases make mistakes
  • Bases are deleted
  • Bases are duplicated

Question 19

Question
How do 6-4 lesions form?
Answer
  • An O is transfered from on thymine to another
  • An N is transfered from on thymine to another
  • A C is transfered from on thymine to another

Question 20

Question
Damage is recognised by bases
Answer
  • True
  • False

Question 21

Question
How does photolyase work on 6-4 bonds?
Answer
  • Uses light to reverse the reaction
  • Uses UV to reverse the reaction
  • Binds to DNA and excises damaged bases

Question 22

Question
A 'protein wedge' forces DNA apart so it can work
Answer
  • True
  • False

Question 23

Question
An e- from diazaflavin is removed by UV and is passed onto what?
Answer
  • FADH
  • NAD
  • NADH

Question 24

Question
The electron will hopefully break the distorted bonds
Answer
  • True
  • False

Question 25

Question
The photolyase process can only work once
Answer
  • True
  • False

Question 26

Question
Which residues bind to the phosphate backbone in photolyase?
Answer
  • Glu
  • Arg
  • Gln

Question 27

Question
What does the widowed base pair bind to?
Answer
  • Water
  • Methyl
  • ATP
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