E.Coli Secretion

Description

Structural Basis for Biological Function (Protein Translocation) Quiz on E.Coli Secretion, created by gina_evans0312 on 21/12/2013.
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Quiz by gina_evans0312, updated more than 1 year ago
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Created by gina_evans0312 over 10 years ago
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Resource summary

Question 1

Question
What is the main problem of protein translocation?
Answer
  • Hydrophillic proteins must transverse two hydrophobic membranes and a hydrophillic space
  • Proteins must be unfolded and refolded again
  • Proteins must pass through an extremely crowded cytoplasm

Question 2

Question
Proteins to be secreted directly after translation have a peptidase sequence at the C-terminus
Answer
  • True
  • False

Question 3

Question
Which of the following parts of the peptidase sequence are closest to the N-terminus?
Answer
  • The basic residues
  • The hydrophobic region
  • Peptidase cleavage recognition site

Question 4

Question
There are 1-2 basic residues in the signal sequence, but the hydrophobic sequence is variable in length
Answer
  • True
  • False

Question 5

Question
When a protein is transcribed with the Peptidase signal site, what protein binds to its hydrophobic region?
Answer
  • Signal Recognition Protein
  • Ftsy- a GTPase
  • SecYEG- a bacterial pore

Question 6

Question
Ftsy transfers the protein into the pore
Answer
  • True
  • False

Question 7

Question
Which of the following provide the driving force for protein translocation across the membrane
Answer
  • Protonmotive force
  • FtsY's GTPase
  • Protein translation

Question 8

Question
What binds to the nascent polypeptide if Post-Translational Translocation takes place?
Answer
  • SecB
  • SecA
  • SecC

Question 9

Question
Post-Translational Translocation sequences have a string of hydrophobic amino acids, same as in Translational Translocation
Answer
  • True
  • False

Question 10

Question
What is the role of Sec_ ?
Answer
  • To keep the protein in solution till it binds to SecA at the pore
  • To fold the protein on its way to the periplasmic more
  • To stop the protein folding on its way to SecC

Question 11

Question
What forces are required to get a Post-Translational Translocation through the pore?
Answer
  • GTPase of FtsY
  • ATP hydrolysis by SecB
  • Protonmotive Force

Question 12

Question
Once inside the required membrane, what protein removes the peptide sequence?
Answer
  • Signal peptidase
  • Signal lyase
  • Signal Isomerase

Question 13

Question
The protein only folds after the signal sequence is cleaved
Answer
  • True
  • False
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