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1781204
Lecture quizzes
Description
A compilation of all of the quizzes from lecture on relevant topics for the final. I forgot to write down the quizzes for topics 25 and 26 though so those are missing.
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bi334
Quiz by
skhal
, updated more than 1 year ago
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Created by
skhal
over 9 years ago
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Resource summary
Question 1
Question
Which property is not essential for the origin of life?
Answer
The need to store information
The need to duplicate information
The need to change information
The need to make proteins
The need to convert into favorable reactions
Question 2
Question
Why is it thought that ribozymes are likely to be a legacy from an ancestral RNA world?
Answer
They are more stable than RNAs
When they act, they require the presence of protein cofactors
They act without the participation of protein cofactors.
They can be made spontaneously without a DNA template.
They are made by proteins
Question 3
Question
What was probably the first carrier of genetic information in primitive organisms?
Answer
DNA that could self-replicate
Proteins
Polypeptides
RNA that could self-replicate
Lipids
Question 4
Question
________ can be used to purify transcription activator proteins
Answer
Gel mobility shift assays
DNA affinity chromatography
Centrifugation
DNA footprinting
ChIP assays
Question 5
Question
Sequence specific DNA binding proteins frequently have a region that binds in the major groove because:
Answer
It is smaller
It contains sequence specific information
The minor groove is not available
It is positively charged
It is negatively charged
Question 6
Question
The following secondary structures are known to specifically interact with DNA (select all that apply):
Answer
Alpha helix
Beta sheet
Unstructured loops
Double helix
Question 7
Question
What happens at the lac operon in the absence of glucose and presence of lactose?
Answer
No expression
Low expression
The lac repressor binds the promoter
High expression
The CAP protein doesn't bind to the promoter
Question 8
Question
Multiple binding sites for a repressor is advantageous because:
Answer
There are too many repressors in cells
DNA must be bent to allow transcription
Repressors are only active when bound to many sites at once
This increases the effective concentration of the repressor
Repressors need multiple binding sites to bind ligands
Question 9
Question
An operator is:
Answer
A protein
A protein binding site
The same as a promoter
A type of repressor
The start site for RNA synthesis
Question 10
Question
Mammalian x-chromosome inactivation:
Answer
Occurs only in humans
Prevents females from being mosaics
Is not necessary
Is necessary for dosage compensation
Only occurs in males
Question 11
Question
CpG islands are generally found in
Answer
Inactive genes
Introns
Exons
Promoters of constantly active genes
Promoters of rarely expressed genes
Question 12
Question
Deamination of methyl-cytosine:
Answer
Does not occur
Changes methyl-cytosine to thymine
Only occurs in the sequence CG
Results in genomic imprinting
Occurs only in expressed genes
Question 13
Question
Sex determination in drosophila is due to:
Answer
Riboswitches
Alternative splicing
Alternative 3' end cleavage
Localization of mRNA
Alternative translational initiation
Question 14
Question
Mirco RNAs (miRNAs):
Answer
Are the same as rRNAs
Are not encoded by the genome
Are found only in bacteria
Are involved in gene repression
Are part of ribosomes
Question 15
Question
Attenuation involves regulation of:
Answer
Translation
Replication
Recombination
Transcription
Proteolysis
Question 16
Question
Ion exchange chromatography separates:
Answer
Proteins, based on size
Proteins, based on surface charge
Proteins, based on source
Question 17
Question
FRET is used to study:
Answer
Protein structure
Protein molecular weight
Protein/protein interaction
Protein sequence
Protein denaturation
Question 18
Question
SDS is used in protein gels to (select all that apply)...
Answer
Make glassware clean up easier
Help denature proteins
To create a uniform mass/charge ratio
To reduce disulfide bonds
Question 19
Question
DNA libraries are used to bank (select all that apply):
Answer
Genomic DNA
RNA
cDNA
Artificial chromosomes
Question 20
Question
Dideoxy nucleotides are used in DNA sequences to:
Answer
Denature the DNA
Hybridize the DNA
Chain terminate the DNA
Elongate the DNA
Transcribe the DNA
Question 21
Question
Hybridization of nucleic acids refers to:
Answer
A renaturation technique
An electrophoretic technique
A cloning technique
A forensic technique
A purification technique
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