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3572623
PCR - Reação em Cadeia da Polimerase
Description
Mind Map on PCR - Reação em Cadeia da Polimerase, created by dehmoraes05 on 25/09/2015.
Mind Map by
dehmoraes05
, updated more than 1 year ago
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Created by
dehmoraes05
almost 9 years ago
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Resource summary
PCR - Reação em Cadeia da Polimerase
Polymerase chain reaction
Amplificação de DNA-Alvo
DNA polimerase termoestável
dNTPS
Etapas:
Desnaturação
Anelamento
Extensão
Sintese de DNA pela Taq polimerase - T 72ºC
Hibridização - Primers iniciadores
Diminuição de temperatura
Altas temperaturas
Separação de dupla fita de DNA
Primers
Termocicladores - automatizam os ciclos de temperatura
Solução para reação
Gera milhões de cópias de fragemnto de DNA conhecido
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