Activation of the RTK


Biochemistry Flowchart on Activation of the RTK, created by Chinazor Okeke on 13/02/2017.
Chinazor Okeke
Flowchart by Chinazor Okeke, updated more than 1 year ago
Chinazor Okeke
Created by Chinazor Okeke almost 7 years ago

Resource summary

Flowchart nodes

  • Activation of Insulin Receptor (RTK)
  • Step 1: Ligand (insulin) binds to RTK 
  • Step 2: Two kinase domains of Beta subunits brought together, auto- crossphosphorylating the tyrosines (Y) of the Beta subunits
  • Step 4: SH2 domain of adj. Insulin Receptor Substrates (IRS-1) now recognizes Pleckstrin domain of PIP2
  • Step 4a: IRS-1 is now phosphorylated an additional three times by RTK, while previous phosphates are removed to conserve Energy
  •  RTK = 2 outside alpha subunit + 2 cytosolic Beta subunits + 1 transmembrane helix domain + extracellular ligand-binding domain
  • Step 4b: Phosphoinositide 3-kinase (PI 3-K) recognizes phosphotyrosine of IRS-1 and binds to become phosphorylated
  • Step 5b: PIP2 phosphorylated to become PIP3 by PI 3-K
  • Step 6b: PIP 3 recruits PDK-1 to membr, activating kinase activity
  • PDK-1 activity is similar to adenyl cyclase b/c both are brought to the membrane
  • Step 7: PDK-1 activates non membrane bound AKT protein (a kinase)
  • Step 8: AKT activated via Pi by hydrolysis of ATP
  • Step 9: AKT activates glycogen synthesis and glucose uptake through crazy phosphorylation!!!
  • Step 3: IRS-1 also separately phosphorylated by RTK
  • Step 10: Glut4 receptors are transported to the cell membrane
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