Created by harrisoncr
over 9 years ago
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Question | Answer |
Name the regions of the electromagnetic spectrum and the colors of the visible spectrum in order of increasing energy and decreasing wavelength. | k |
State Beer's law in terms of absorbance and concentration and apply it in quantitative calculations. | k |
Diagram and explain the purpose of each component of a spectrophotometer: -Light Source | k |
Diagram and explain the purpose of each component of a spectrophotometer: -Monochromator | d |
Diagram and explain the purpose of each component of a spectrophotometer: -Sample Holder | l |
Diagram and explain the purpose of each component of a spectrophotometer: -Detector | lk |
Diagram and explain the purpose of each component of a spectrophotometer: -Readout Device | l |
Explain the term matrix and the use of a blank. | l |
Give an example of a signal amplifier. | l |
List the similarities and differences between prisms and diffraction grating. | l |
Compare single and double beam. | l |
Explain the relationship between: -Color and Wavelength | f |
Explain the relationship between: -Absorbance and Concentration | l |
Explain the relationship between: -Absorbance and Transmission | s |
Differentiate between light emission, reflection, and transmittance. | l |
Colorimetry | l |
Photometry | l |
Spectrophotometer | l |
λ | l |
Frequency | l |
Absorbance | l |
Transmission | l |
Describe the principle of fluorometry and the basic components of a fluorometer. Explain why fluorescence measurements may be more specific and sensitive than spectrophotometric measurements. | l |
List the different types of chromatography and the basis for separation used in each. | j |
Explain the principle of gas-liquid chromatography and describe several different types of detectors. | j |
Describe the principle of electrophoresis and explain how it is commonly used in a clinical laboratory. | l |
Describe the principle and components of a pCO2 electrode. | l |
Discuss the principle of an ion-selective electrode technology and list the ions commonly measured by it in a clinical laboratory. | l |
Describe common features found within automated instruments. | l |
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