MEDT 2410: Chapter 5 Lesson Objectives

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College MEDT 2410: Clinical Chemistry Flashcards on MEDT 2410: Chapter 5 Lesson Objectives, created by harrisoncr on 27/08/2015.
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Flashcards by harrisoncr, updated more than 1 year ago
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Created by harrisoncr over 8 years ago
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Question Answer
Name the regions of the electromagnetic spectrum and the colors of the visible spectrum in order of increasing energy and decreasing wavelength. k
State Beer's law in terms of absorbance and concentration and apply it in quantitative calculations. k
Diagram and explain the purpose of each component of a spectrophotometer: -Light Source k
Diagram and explain the purpose of each component of a spectrophotometer: -Monochromator d
Diagram and explain the purpose of each component of a spectrophotometer: -Sample Holder l
Diagram and explain the purpose of each component of a spectrophotometer: -Detector lk
Diagram and explain the purpose of each component of a spectrophotometer: -Readout Device l
Explain the term matrix and the use of a blank. l
Give an example of a signal amplifier. l
List the similarities and differences between prisms and diffraction grating. l
Compare single and double beam. l
Explain the relationship between: -Color and Wavelength f
Explain the relationship between: -Absorbance and Concentration l
Explain the relationship between: -Absorbance and Transmission s
Differentiate between light emission, reflection, and transmittance. l
Colorimetry l
Photometry l
Spectrophotometer l
λ l
Frequency l
Absorbance l
Transmission l
Describe the principle of fluorometry and the basic components of a fluorometer. Explain why fluorescence measurements may be more specific and sensitive than spectrophotometric measurements. l
List the different types of chromatography and the basis for separation used in each. j
Explain the principle of gas-liquid chromatography and describe several different types of detectors. j
Describe the principle of electrophoresis and explain how it is commonly used in a clinical laboratory. l
Describe the principle and components of a pCO2 electrode. l
Discuss the principle of an ion-selective electrode technology and list the ions commonly measured by it in a clinical laboratory. l
Describe common features found within automated instruments. l
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