Secretion and Adhesion System Questions

Cholera toxin is secreted by a mechanism that mimics what process

Which secretion system has a needle complex for injection of bacterial toxins into the host cell?

Which species injects a receptor for the adhesin intimin into the host cell?

How does Shigella enter the host cell?

Adherence Type I: [blank_start]SecA-dependent[blank_end] Type IV: [blank_start]SecA- independent[blank_end]

Match the category of exotoxin to the correct example. [blank_start]Plasma membrane disruption[blank_end] Staphylococcus aureus alpha toxin [blank_start]Cytoskeleton alterations[blank_end] Vibrio cholerae RTX toxin [blank_start]Protein synthesis disruption[blank_end] Diphtheria toxin [blank_start]Cell cycle disruption[blank_end] E. coli cytolethal distending toxin [blank_start]Signal transduction disruption[blank_end] Cholera toxin [blank_start]Cell-cell adherence[blank_end] Staphylococcus aureus exfoliative toxin [blank_start]Vesicle traffic[blank_end] Helicobacter pylori VacA toxin [blank_start]Exocytosis[blank_end] Tetanus toxin [blank_start]Superantigens[blank_end] Staphylococcus auerus and Streptococcus pyogenes pyrogenic toxins

Which secretion system uses a conjugation-like machinery to secrete protein from cytoplasm or periplasm?

Which secretion system uses molecular syringe to inject proteins from bacterial cytoplasm into host cytoplasm?

What type of secretion system uses pilus-like extraction/retraction mechanism to push proteins out of the cell

[blank_start]Cilia[blank_end], [blank_start]fimbriae[blank_end], and [blank_start]pili[blank_end] are all examples of structures used by microbes for adhesion.

You have recently identified a new toxin. It is produced by a gram-negative bacterium. It is composed mostly of protein, has high toxicity, and is not heat stable. You also discover that it targets liver cells. Based on these characteristics, how would you classify this toxin?

You have recently identified a new toxin. It is produced by a gram-negative bacterium. It is composed mostly of polysaccharides, binds to immune cells, and triggers fevers. Based on these characteristics, how would you classify this toxin?

Match the toxin to its secretion system. [blank_start]Salmonella typhimurium[blank_end] [blank_start]Type III[blank_end] Uses molecular syringe to inject proteins from bacterial cytoplasm into host cytoplasm [blank_start]Pertussis toxin[blank_end] [blank_start]Type IV[blank_end] Uses a conjugation-like machinery to secrete protein from cytoplasm or periplasm [blank_start]Cholera toxin[blank_end] [blank_start]Type II[blank_end] Uses pilus-like extraction/retraction mechanism to push proteins out of the cell [blank_start]E. coli alpha hemolysin[blank_end] [blank_start]Type I[blank_end] Uses ABC mechanisms that move certain secreted proteins directly out of the cell

4. Which of the following pathogens does not have endotoxin?

If SecA were mutated, E. coli could adhere to renal cells but would be unable to secrete exotoxins to cause infection.

15. How does the plague bacterium survive the immune response?

You observe a protein structure that has two subunits and is involved in bacterial pathogenesis. This protein is most likely which of the following?