7282123
Description
Mind Map by Deleted user, updated more than 1 year ago
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Created by a deleted user
over 7 years ago
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Copied by a deleted user
over 7 years ago
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Polymorphism Detection
- Transcription
- RNA
- Northern Blot
- Materials
- Total mRNA fraction
- Agarose gel
- Filter
- Radioactive probes of ssDNA
complimentary to mRNA of interst
- Total mRNA fraction
- Steps
- Isolate mRNA
- Electrophoresis of RNA sample
- smear of RNA
- Transfer RNA to filter
- Incubate filter with radioactive probe
- Incubate filter with radioactive probe
- smear of RNA
- Electrophoresis of RNA sample
- Isolate mRNA
- Materials
- Northern Blot
- RNA
- DNA
- Detect SNPs in DNA
- Sanger sequencing
- ddNTP
- ddNTP
- Restriction Fragment Length Polymorphisms (RFLP)
- Materials
- DNA of interest
- Restriction enzymes
- cut at restriction sites
- cut at restriction sites
- Buffer
- maintain optimal conditions
- maintain optimal conditions
- DNA of interest
- Steps
- Add materials
- incubate tube for X min at Y temp
- Add materials
- How to detect SNP
- seq has SNP that causes restriction site/target site
- Therefore, cut DNA in more places
- More smaller fragments
- Electrophoresis on gel
- Southern Blot
- RFLP with radio-labeled DNA
- After restriction
fragments run
out on gel
- products are denatured (dsDNA to ssDNA)
- Transfer to filter
- Put filter in bag with radio-labeled
nucleic acid probe
- Expose X-ray film to membrane
- Expose X-ray film to membrane
- Put filter in bag with radio-labeled
nucleic acid probe
- Transfer to filter
- products are denatured (dsDNA to ssDNA)
- SNPs or SSLPs
- RFLP with radio-labeled DNA
- DNA must be stained to see
- Thicker: 2x amount
- Thicker: 2x amount
- Southern Blot
- Electrophoresis on gel
- More smaller fragments
- Therefore, cut DNA in more places
- seq has SNP that causes restriction site/target site
- Materials
- Microarray
- computer
compares multiple
sequences at once
- computer
compares multiple
sequences at once
- PCR
- stain and electrophoresis
- stain and electrophoresis
- Sanger sequencing
- Detect SNPs in DNA
- Translation
- Protein
- Western Blot
- Materials
- Protein of interest
- Polyacrylamide gel
- smaller pores than agrose
- smaller pores than agrose
- filter
- antibody
- protein that binds to protein of interest
- protein that binds to protein of interest
- Protein of interest
- Steps
- Isolate all proteins
- Run proteins on gel
- transfer to filter
- incubate filter with radioactive
probe
- Isolate all proteins
- Materials
- Western Blot
- Protein
Media attachments
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