Th1L07 Enzymes

1. reduces necessary free energy (delta G) need to overcome the activation energy

1. Dependent on many weak bonds e.g. hydrogen bonds, electrostatic salt links and hydrophobic interactions
   1. Makes enzymes sensitive to changes in their environment
      1. Inactive enzyme
         1. Denatured enzyme state
      2. e.g. by eating the protein, giving rise to a disorganised or tangled structure in which the enzyme no longer has any catalytic activity
      3. temperature
         1. Between 20C, 30C and 40C for enzymes found in the body, you will usually speed up the reaction
         2. Up to 50C and beyond, denaturing of the protein will occur, similarly going to far below 20C will also reduce reaction velocity

1. illustrates that enzymes should be complementary to the substrates transition phase rather than the substrate itself to bring about the activation of the reaction > product

1. few are so specific that they will act only on a certain enzyme even to the point will it will only act on one stereoisomer

1. multiple enzymes within the same cellular compartment can co-exist without interfering with e/o
   1. allows for multiple metabolic pathways w/i cells

Annotations:

• Six classes are divided into subgroups according to their substrate or source i.e. the type of reaction they catalyse                               
• Each enzyme IDed by individual 4 digit number        I.U.B. Commission on Enzymes - established the systematic classification on enzymes           Catalase 4 digit classification code - E.C. 1.11.1.6.          

1. mnemonic: TO HILL
   1. transferase
   2. oxoreductase
   3. hydrolyase
   4. isomerase
   5. ligase
   6. lyases