22499279
Description
Flashcards by Evian Chai, updated more than 1 year ago
|
Created by Evian Chai
about 4 years ago
|
|
| Question | Answer |
| What is KCat? | The turnover number: number of substrate molecules converted to products in a certain amount of time |
| What is the substrate vs reaction graph? How does it change as the concentration increases | Concentration of substrate on the x axis against rxn At low concentrations, substrate proportional to rxn rate: 1st order At high concentrations, rxn rate independent of substrate: 0 order |
| What is the Michaelis Menton reaction? What do K1, K-1, and K2 stand for? | E+S<-->ES-->E+P K1: E+S-->ES K-1: E+S<--ES K2: ES--> E+P |
| What are 5 assumptions of the Michaelis Menton reaction? | 1. [substrate]>>>[enzyme] 2. [ES] doesn't change 3. Initial velocity used 4. P is small enough to be negligible 5. ES<--E+P ignored |
| What is the Michaelis Menton equation? | V= Vmax[S]/Km+[S] |
| What does Km represent in terms of Vmax? What about affinity? | It is the concentration of substrate when Vmax 1/2. A lower Km indicates a higher affinity |
| K2....K-1 so rxn to E+P neglibale | K2<<<<K-1 |
| What is Kd? | The dissociation constant of ES aka K2 |
| What do phosphorylases do? | Transfer phosphate group from inorganic molecule to molecule |
| How does protein phosphorylation affect glycogen phosphorylase and glycogen synthase? | Phosphorylation activates glycogen phosphorylase and inactivates glycogen synthase |
| What does phosphatase do? | Uses H20 to cleave Phosphate from molecule |
| On a graph, what does a competitive inhibitor change and not change? What about noncompetitive inhibitor? | -Increases Km but not Vmax bc can be overcome -Decreases Vmax but not Km |
| What is the difference between allosteric and noncompetitive enzymes? | Noncompetitive poisons/damages enzyme |
| What is an example of negative allosteric regulation on phosphofructokinase? | ATP/Citrate on Phosphofructokinase As a result, Fructose 6 phosphate does not become Fructose 1,6 biphosphate |
| What is an example of positive allosteric regulation on pyruvate kinase? | Fructose 1,6-biphosphate and phosphoenol pyruvate increase its activity More conversion of phosphoenol pyruvate-->pyruvate as a result |
| On the Lineweaver Burke plot, the Y intercept represents.... The X intercept represents.... | Y intercept: 1/Vmax (changes w allosteric) X intercept: -1/Km (changes w competitive) |
| What is an example of a isoenzyme? | Lactate dehydrogenase- has 4 monomers Each one can be heart/muscle type |
Want to create your own Flashcards for free with GoConqr? Learn more.