1822271
Description
Flashcards by barker.i.natalie, updated more than 1 year ago
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Created by barker.i.natalie
over 9 years ago
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| Question | Answer |
| Amplification Curve | Fluorescence is proportional to the amount of product 1. Ct signal important (cycle threshold) 2. Ct low - more DNA present (Fewer amplification cycles to rise above threshold) 3. Homozygote: 1 probe binds, all DNA contributes to an increase in fluorescence 4. Heterozygote: 1/2 DNA probe 1 1/2 DNA probe 2 - Signal for each will be higher than homozygote signal |
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