Let's Learn Agars

Question 1 of 20

1

Indole:
Substrate: ❌, Enzyme: ❌, Detection Reagent: ❌
Positive Reaction: ❌, Negative Reaction: ❌
End Product: Indole

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Trypotophan

Glucose

Gelatin

Typtophanase

Fermentation

Nitrate

Kovac's

Methyl Red

Decarboxylase

Pink ring

Green ring

Purple

Yellow Ring

Explanation

Question 2 of 20

1

Methyl Red
Substrate: ❌, Enzyme: ❌, Detection Reagent: ❌
Positive: ❌, Negative: ❌
End Product: Mixed Acids

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Glucose

Typtophan

Individual carbohydrate & pepton

Fermentation

Decarboxylase

Permeoase

Methyl Red

Phenyl Red

Red

Blue

Black

Yellow

Yellow ring

blue

Explanation

Question 3 of 20

1

Voges-Proskauer
Substrate: ❌, Enzyme: ❌, Detection Reagent: ❌
Positive: ❌, Negative: ❌
End-Product: Acetoin

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Glucose

Semi-Solid Agar

Lysine

Fermentation

Urease

Deaminase or Decarboxylase

Alpha Naptol & KOH

TTC

None

Red

Yellow

No Color Change

Pink Ring

Explanation

Question 4 of 20

1

Citrate
Substrate: ❌, Enzyme: ❌, Detection Reagent: ❌
Postive: ❌, Negative: ❌
End Product: Sodium Carbonate

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Sodium Citrate

Citric Acid

Amino Acid

Citrate Permease

Amino Acid Decarboxylase

Phenylalanine deaminase

Bronthymol Blue

Bromine Sol Purple

Ferric Chloride

Blue

Violet

Green

Yellow

No color change

Explanation

Question 5 of 20

1

Decarboxylation
Substrate: ❌, Enzyme: ❌, Detection Reagent: ❌
Positive: ❌, Negative: ❌
End Product: Primary amine for amino acid

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Amino Acid

Sodium Citrate

Phenylalanine

Glucose

Amino Acid Decarboxylase

No enzyme

Bromocresol Purple

Bronthymol Blue

Phenol Red

Purple

Pink Ring

Yellow

Yellow Ring

Green

Explanation

Question 6 of 20

1

Deamination Phenylalanine or TDA
Substrate: ❌, Enzyme: ❌, Detection Reagent: ❌
Positive: ❌, Negative: ❌ (Hint: color of the slant)
End Product: Phenyl Pyruvic Acid

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Phenylalanine

Amino Acid

Sodium Citrate

Glucose & Lysine

Phenylalanine Deaminase

No enzyme

Decarboxylase

Ferric Chloride

Bromocresol Purple

Bromthymol Blue

Green

Black

Red

Yellow

Pink

Explanation

Question 7 of 20

1

Gelatin
Substrate: ❌, Enzyme: ❌, Detection Reagent: ❌
Positive: ❌, Negative: ❌
End Product: Liquid or Solid

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Gelatin

Peanut butter

Glucose

Amino Acid

Gelatinase

Decarboxylase

Deaminase

Beta galactosidase

None

Methyl Red

Sulfanilic acid & alpha napthylamine

Liquid

Red

Yellow ring

Solid

Black

Explanation

Question 8 of 20

1

ONPG
Substrate: ❌, Enzyme: ❌, Detection Reagent: ❌
Positive: ❌, Negative: ❌
End Product: Ortho Nitrophenol

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ONPG

Nitrate

Individual Carbohydrate & pepton

Beta Galactosidase

No enzyme

Amino Acid Decarboxylase

Chromogenic Compound

Bromothymol Blue

Ferric Chloride

Yellow

Pink

Green slant

Clear

Purple

Explanation

Question 9 of 20

1

Nitrate
Substrate: ❌, Enzyme: ❌, Detection Reagent: ❌
Positive: ❌ after reagent, colorless after zinc, Negative: Colorless after reagents, Red after zinc
End Product: N/A

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Nitrate

No need to study this one

Nitrate & or Nitrite redcutase

lalalala

Sulfanilic Acid

Chocolate

Red

Rainbow

Glittery

Explanation

Question 10 of 20

1

Lysine Iron Agar
Substrate: ❌, Enzyme: ❌, Detection Reagent: ❌
Positive: ❌, Negative: ❌ (and black is H2S)
End Product: Ferric Ammonium Citrate

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Glucose & Lysine

Glucose only

Semi-solid Agar

ONPG

Deaminase &/or Decarboxylase

No enzyme

Urease

Bromocresol Purple

Bromothymol Blue

Phenol Red

Alkaline

Liquid

Purple

Acid

Solid

Black

Explanation

Question 11 of 20

1

Motility (Semi-Solid)
Substrate: ❌, Enzyme:❌ , Detection Reagent: ❌
Positive: ❌, Negative: ❌
End Product: Examine growth closely

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Semi-solid agar

Gelatin

Glucose

No enzyme

Typtophan

Decarboxylase

Citrate

TTC

Phenol Red

Ferric Acid

Motile

Solid

Liquid

Non-Motile

Yellow

Explanation

Question 12 of 20

1

Urea
Substrate: ❌, Enzyme: ❌, Detection Reagent: ❌
Positive: ❌, Negative: ❌ (Hint: Sometimes only slant changes color)
End Product: H2O + CO2

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Urea

Glucose

Lysine

Citrate

Urease

No enzyme

Deaminase

Phenol Red

Bromothymol Blue

Ferric Chloride

Kovac's

Pink

Black

Deep Orange

Yellow

Green

Explanation

Question 13 of 20

1

Carbohydrate Fermentation
Substrate: ❌, Enzyme: ❌, Detection Reagent: ❌
Positive: ❌, Negative: Same
End Product: Acid &/or Gas

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Individual Carbohydrate & pepton

Glucose

Amino Acid

No enzyme

Deaminase

Citrate permease

Different Ph Indicators

Butterflies

Color changes with indicator

Explanation

Question 14 of 20

1

Select from the dropdown lists to complete the text.

For TSI/KIA
K/A indicated that ( Only glucose, Glucose & Lactose, No sugar ) has been fermented
Gas ( has not, has ) been fermented

Explanation

Question 15 of 20

1

Select from the dropdown lists to complete the text.

For TSI/ KIA
K/(A) or K/Ag ( only glucose, glucose and lactose, No sugar ) has been fermented
( With, No ) gas production

Explanation

Question 16 of 20

1

Select from the dropdown lists to complete the text.

For TSI/ KIA
K/(A)h2sor K/Ag+++ ( Only glucose, No sugar, Glucose and lactose ) has been fermented
( With, no ) gas production
( With, no ) H2s Production

Explanation

Question 17 of 20

1

Select from the dropdown lists to complete the text.

For TSI/KIA
K/Ah2s or K/A ( only glucose, Glucose and lactose, No sugar ) has been fermented
( No, With ) gas production
( With, No ) H2s production

Explanation

Question 18 of 20

1

Select from the dropdown lists to complete the text.

For TSI/KIA
A/(A) or A/Ag ( Both glucose and lactose, No sugar, Only Glucose )(possibly sucrose) has been fermented
( With, No ) gas production
( No, With ) H2S Production

Explanation

Question 19 of 20

1

Select from the dropdown lists to complete the text.

For TSI/KIA
A/(A)h2s or A/Ag+++ ( Both glucose and lactose, Only glucose, No sugar ) has been fermented
( With, No ) gas production
( With, No ) H2S production

Explanation

Question 20 of 20

1

	Created by Katie Koontz about 8 years ago

All about agars

Let's Learn Agars

Let's Learn Agars

Question 1 of 20

Indole: Substrate: ❌, Enzyme: ❌, Detection Reagent: ❌ Positive Reaction: ❌, Negative Reaction: ❌ End Product: Indole

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Explanation

Question 2 of 20

Methyl Red Substrate: ❌, Enzyme: ❌, Detection Reagent: ❌ Positive: ❌, Negative: ❌ End Product: Mixed Acids

Drag and drop to complete the text.

Explanation

Question 3 of 20

Voges-Proskauer Substrate: ❌, Enzyme: ❌, Detection Reagent: ❌ Positive: ❌, Negative: ❌ End-Product: Acetoin

Drag and drop to complete the text.

Explanation

Question 4 of 20

Citrate Substrate: ❌, Enzyme: ❌, Detection Reagent: ❌ Postive: ❌, Negative: ❌ End Product: Sodium Carbonate

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Explanation

Question 5 of 20

Decarboxylation Substrate: ❌, Enzyme: ❌, Detection Reagent: ❌ Positive: ❌, Negative: ❌ End Product: Primary amine for amino acid

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Explanation

Question 6 of 20

Deamination Phenylalanine or TDA Substrate: ❌, Enzyme: ❌, Detection Reagent: ❌ Positive: ❌, Negative: ❌ (Hint: color of the slant) End Product: Phenyl Pyruvic Acid

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Explanation

Question 7 of 20

Gelatin Substrate: ❌, Enzyme: ❌, Detection Reagent: ❌ Positive: ❌, Negative: ❌ End Product: Liquid or Solid

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Explanation

Question 8 of 20

ONPG Substrate: ❌, Enzyme: ❌, Detection Reagent: ❌ Positive: ❌, Negative: ❌ End Product: Ortho Nitrophenol

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Explanation

Question 9 of 20

Nitrate Substrate: ❌, Enzyme: ❌, Detection Reagent: ❌ Positive: ❌ after reagent, colorless after zinc, Negative: Colorless after reagents, Red after zinc End Product: N/A

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Explanation

Question 10 of 20

Lysine Iron Agar Substrate: ❌, Enzyme: ❌, Detection Reagent: ❌ Positive: ❌, Negative: ❌ (and black is H2S) End Product: Ferric Ammonium Citrate

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Explanation

Question 11 of 20

Motility (Semi-Solid) Substrate: ❌, Enzyme:❌ , Detection Reagent: ❌ Positive: ❌, Negative: ❌ End Product: Examine growth closely

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Explanation

Question 12 of 20

Urea Substrate: ❌, Enzyme: ❌, Detection Reagent: ❌ Positive: ❌, Negative: ❌ (Hint: Sometimes only slant changes color) End Product: H2O + CO2

Drag and drop to complete the text.

Explanation

Question 13 of 20

Carbohydrate Fermentation Substrate: ❌, Enzyme: ❌, Detection Reagent: ❌ Positive: ❌, Negative: Same End Product: Acid &/or Gas

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Explanation

Question 14 of 20

Select from the dropdown lists to complete the text.

For TSI/KIA K/A indicated that Only glucose Glucose & Lactose No sugar( Only glucose, Glucose & Lactose, No sugar ) has been fermented Gas has not has( has not, has ) been fermented

Explanation

Question 15 of 20

Select from the dropdown lists to complete the text.

For TSI/ KIA K/(A) or K/Ag only glucose glucose and lactose No sugar( only glucose, glucose and lactose, No sugar ) has been fermented With No( With, No ) gas production

Explanation

Question 16 of 20

Select from the dropdown lists to complete the text.

For TSI/ KIA K/(A)h2sor K/Ag+++ Only glucose No sugar Glucose and lactose( Only glucose, No sugar, Glucose and lactose ) has been fermented With no( With, no ) gas production With no( With, no ) H2s Production

Explanation

Question 17 of 20

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For TSI/KIA K/Ah2s or K/A only glucose Glucose and lactose No sugar( only glucose, Glucose and lactose, No sugar ) has been fermented No With( No, With ) gas production With No( With, No ) H2s production

Explanation

Question 18 of 20

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For TSI/KIA A/(A) or A/Ag Both glucose and lactose No sugar Only Glucose( Both glucose and lactose, No sugar, Only Glucose )(possibly sucrose) has been fermented With No( With, No ) gas production No With( No, With ) H2S Production

Explanation

Question 19 of 20

Select from the dropdown lists to complete the text.

For TSI/KIA A/(A)h2s or A/Ag+++ Both glucose and lactose Only glucose No sugar( Both glucose and lactose, Only glucose, No sugar ) has been fermented With No( With, No ) gas production With No( With, No ) H2S production

Explanation

Question 20 of 20

Indole:
Substrate: ❌, Enzyme: ❌, Detection Reagent: ❌
Positive Reaction: ❌, Negative Reaction: ❌
End Product: Indole

Methyl Red
Substrate: ❌, Enzyme: ❌, Detection Reagent: ❌
Positive: ❌, Negative: ❌
End Product: Mixed Acids

Voges-Proskauer
Substrate: ❌, Enzyme: ❌, Detection Reagent: ❌
Positive: ❌, Negative: ❌
End-Product: Acetoin

Citrate
Substrate: ❌, Enzyme: ❌, Detection Reagent: ❌
Postive: ❌, Negative: ❌
End Product: Sodium Carbonate

Decarboxylation
Substrate: ❌, Enzyme: ❌, Detection Reagent: ❌
Positive: ❌, Negative: ❌
End Product: Primary amine for amino acid

Deamination Phenylalanine or TDA
Substrate: ❌, Enzyme: ❌, Detection Reagent: ❌
Positive: ❌, Negative: ❌ (Hint: color of the slant)
End Product: Phenyl Pyruvic Acid

Gelatin
Substrate: ❌, Enzyme: ❌, Detection Reagent: ❌
Positive: ❌, Negative: ❌
End Product: Liquid or Solid

ONPG
Substrate: ❌, Enzyme: ❌, Detection Reagent: ❌
Positive: ❌, Negative: ❌
End Product: Ortho Nitrophenol

Nitrate
Substrate: ❌, Enzyme: ❌, Detection Reagent: ❌
Positive: ❌ after reagent, colorless after zinc, Negative: Colorless after reagents, Red after zinc
End Product: N/A

Lysine Iron Agar
Substrate: ❌, Enzyme: ❌, Detection Reagent: ❌
Positive: ❌, Negative: ❌ (and black is H2S)
End Product: Ferric Ammonium Citrate

Motility (Semi-Solid)
Substrate: ❌, Enzyme:❌ , Detection Reagent: ❌
Positive: ❌, Negative: ❌
End Product: Examine growth closely

Urea
Substrate: ❌, Enzyme: ❌, Detection Reagent: ❌
Positive: ❌, Negative: ❌ (Hint: Sometimes only slant changes color)
End Product: H2O + CO2

Carbohydrate Fermentation
Substrate: ❌, Enzyme: ❌, Detection Reagent: ❌
Positive: ❌, Negative: Same
End Product: Acid &/or Gas

For TSI/KIA
K/A indicated that ( Only glucose, Glucose & Lactose, No sugar ) has been fermented
Gas ( has not, has ) been fermented

For TSI/ KIA
K/(A) or K/Ag ( only glucose, glucose and lactose, No sugar ) has been fermented
( With, No ) gas production

For TSI/ KIA
K/(A)h2sor K/Ag+++ ( Only glucose, No sugar, Glucose and lactose ) has been fermented
( With, no ) gas production
( With, no ) H2s Production

For TSI/KIA
K/Ah2s or K/A ( only glucose, Glucose and lactose, No sugar ) has been fermented
( No, With ) gas production
( With, No ) H2s production

For TSI/KIA
A/(A) or A/Ag ( Both glucose and lactose, No sugar, Only Glucose )(possibly sucrose) has been fermented
( With, No ) gas production
( No, With ) H2S Production

For TSI/KIA
A/(A)h2s or A/Ag+++ ( Both glucose and lactose, Only glucose, No sugar ) has been fermented
( With, No ) gas production
( With, No ) H2S production

For TSI/KIA
K/K ( No sugar, Only glucose, Both glucose and lactose ) has been fermented
( No, With ) gas production
( No, With ) H2s production