Natural Products

Question 1 of 13

1

In specimen collection the plant must be dried and kept sterile to minimise damage

Select one of the following:

True
False

Explanation

Question 2 of 13

1

Which of the following is best to aid identification of the natural product/plant

Select one of the following:

plant handbook
flora or field guide
herbarium

Explanation

Question 3 of 13

1

macerated leaves are treated with chemicals that gradually decrease in polarity to extract chemicals

Select one of the following:

True
False

Explanation

Question 4 of 13

1

Select from the dropdown lists to complete the text.

General screening bioassays are ( simple, complex ), ( inexpensive, expensive ) and used for ( rapid screening, specific enzyme screening ) of extracts

Explanation

Question 5 of 13

1

Select from the dropdown lists to complete the text.

specialised screening bioassays are ( subcellular, multicellular ) assays directed at ( specific, broad spectrum ) enzymes or receptors. Compounds working by mechanisms unrelated to the assay are likely to be missed.

Explanation

Question 6 of 13

1

which of the following is FALSE

Select one of the following:

Brine shrimp lethality tests are used to determine cytotoxicity (potential antitumour) of extracts
crown gall tumour inhibition tests give a fairly accurate prediction of in vivo murine antileukemic activity
starfish or sea urchin assays are used to investigate in vivo antileukemic activity
agar dilution streak assay means up to 7 microorganisms can be screened simultaneously on a petri dish
microtitre plates allow testing of 3 dilutions of one or two extracts against up to 24 microorganisms

Explanation

Question 7 of 13

1

crown gall tumour inhibition tests use potatoes to measure inhibition of growth

Select one of the following:

True
False

Explanation

Question 8 of 13

1

which 3 do starfish or sea urchin assays allow detection of?

Select one or more of the following:

dna and rna synthesis inhibitors
mRNA substrate inhibitors
microtubule assembly inhibitors
protein synthesis inhibitors

Explanation

Question 9 of 13

1

Click on the orange point and select the correct answer.

which best applies to an agar dilution streak assay?

Explanation

Question 10 of 13

1

Click on the orange point and select the correct answer.

Which tlc staining technique is used to test acidic groups

Explanation

Question 11 of 13

1

which one of the following is not considered in NP isolation or synthesis

Select one of the following:

source - availability
environmental and local impact
cost
complexity of novel structures
diluent needed

Explanation

Question 12 of 13

4

Select from the dropdown lists to complete the text.

What are the stages of natural product processing? ( Specimen, Taxonomy, molecules, structure ) collection, establishing ( taxonomy, specimen, molecules, therapeutics ), extracting possible active molecules, using ( screening, showing, therapeutic, herbariums ) techniques to evaluate therapeutic activity, identifying and ( isolating, burning, melting, releasing ) the structure responsible for the activity, using organic synthesis

Explanation

Question 13 of 13

2

Select from the dropdown lists to complete the text.

a range of techniques are used to isolate active compounds (fractionation). these include ( TLC, LTC, MSMS ), column chromatography, ( HPLC, ELISA, SPE ) and combinations such as HPLC/MS

	Created by Sam Watkins about 8 years ago

a quiz on natural product extraction and tests

Natural Products

Natural Products

Question 1 of 13

In specimen collection the plant must be dried and kept sterile to minimise damage

Select one of the following:

Explanation

Question 2 of 13

Which of the following is best to aid identification of the natural product/plant

Select one of the following:

Explanation

Question 3 of 13

macerated leaves are treated with chemicals that gradually decrease in polarity to extract chemicals

Select one of the following:

Explanation

Question 4 of 13

Select from the dropdown lists to complete the text.

General screening bioassays are simple complex( simple, complex ), inexpensive expensive( inexpensive, expensive ) and used for rapid screening specific enzyme screening( rapid screening, specific enzyme screening ) of extracts

Explanation

Question 5 of 13

Select from the dropdown lists to complete the text.

specialised screening bioassays are subcellular multicellular( subcellular, multicellular ) assays directed at specific broad spectrum( specific, broad spectrum ) enzymes or receptors. Compounds working by mechanisms unrelated to the assay are likely to be missed.

Explanation

Question 6 of 13

which of the following is FALSE

Select one of the following:

Explanation

Question 7 of 13

crown gall tumour inhibition tests use potatoes to measure inhibition of growth

Select one of the following:

Explanation

Question 8 of 13

which 3 do starfish or sea urchin assays allow detection of?

Select one or more of the following:

Explanation

Question 9 of 13

Click on the orange point and select the correct answer.

which best applies to an agar dilution streak assay?

Explanation

Question 10 of 13

Click on the orange point and select the correct answer.

Which tlc staining technique is used to test acidic groups

Explanation

Question 11 of 13

which one of the following is not considered in NP isolation or synthesis

Select one of the following:

Explanation

Question 12 of 13

Select from the dropdown lists to complete the text.

Explanation

Question 13 of 13

Select from the dropdown lists to complete the text.

a range of techniques are used to isolate active compounds (fractionation). these include TLC LTC MSMS( TLC, LTC, MSMS ), column chromatography, HPLC ELISA SPE( HPLC, ELISA, SPE ) and combinations such as HPLC/MS

Explanation

General screening bioassays are ( simple, complex ), ( inexpensive, expensive ) and used for ( rapid screening, specific enzyme screening ) of extracts

specialised screening bioassays are ( subcellular, multicellular ) assays directed at ( specific, broad spectrum ) enzymes or receptors. Compounds working by mechanisms unrelated to the assay are likely to be missed.

a range of techniques are used to isolate active compounds (fractionation). these include ( TLC, LTC, MSMS ), column chromatography, ( HPLC, ELISA, SPE ) and combinations such as HPLC/MS