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Structural Basis for Biological Function (Membrane Proteins) Quiz on Oxidation Practical, created by gina_evans0312 on 05/01/2014.

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Oxidation Practical

Question 1 of 8

1

What is the difference between Stage 3 & Stage 4?

Select one or more of the following:

  • Stage 3 is between ADP addition and ADP running out

  • Stage 3 is between ADP running out and oxygen running out

  • Stage 4 is between ADP running out and oxygen running out

  • Stage 4 is between O2 running out and ADP running out

Explanation

Question 2 of 8

1

Measuring oxygen consumption is done by looking at the change in the X co-ordinates

Select one of the following:

  • True
  • False

Explanation

Question 3 of 8

1

Once you've worked out the change in O2 concentraction, how do you calculate nmol O2 /min/mg protein

Select one of the following:

  • Divide by mg of protein

  • Divide by 60

  • Divide by O2 consumed

Explanation

Question 4 of 8

1

Assume a change in the axis of 15mm, and a protein concentration of 1.4mg. Calculate the Rate

Select one of the following:

  • 25.7 nm Oxygen/min/mg protein

  • 10.7 nm Oxygen/min/mg protein

  • 50.4 nm Oxygen/min/mg protein

Explanation

Question 5 of 8

1

What is the equation for the respiratory control ratio?

Select one of the following:

  • Rate of phase 4/ Rate of phase 3

  • Rate of phase 3/ Rate of phase 4

  • Rate of phase 3 x Rate of phase 4

Explanation

Question 6 of 8

1

ADP/O ratio = Moles of ADP added/Moles of ADP consumed

Select one of the following:

  • True
  • False

Explanation

Question 7 of 8

1

To convert from O2 to O, what must you do?

Select one of the following:

  • Double it

  • Halve it

  • Square it

Explanation

Question 8 of 8

1

What must be checked before calculating the ADP/O ratio?

Select one of the following:

  • That both sides are in the same molar concentration

  • That the oxygen consumed has been converted into nm

  • That the ratio is positive

Explanation

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