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Structural Basis for Biological Function (DNA-Protein Interactions) Quiz on Protein-DNA Interactions- DNA Repair, created by gina_evans0312 on 24/12/2013.

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Protein-DNA Interactions- DNA Repair

Protein-DNA Interactions- DNA Repair

Question 1 of 27

1

Which of the following are caused by hydrolysis?

Select one or more of the following:

  • Nicks

  • Abasic Sites

  • Cross Links

  • Ds Breaks

Explanation

Question 2 of 27

1

Which of the following are caused by Radical Oxygen Species?

Select one or more of the following:

  • Bulky alkylation damage

  • Abasic sites

  • ds breas

  • ss breaks

  • Nicks

Explanation

Question 3 of 27

1

What causes Alkylation Damage?

Select one or more of the following:

  • S-adenosyl methionine

  • Radical oxygen species

  • Interacts with 6th O on guanine

  • Interacts with 3rd O on guanine

Explanation

Question 4 of 27

1

Methylation of guanine results in unstable base pairing

Select one of the following:

  • True
  • False

Explanation

Question 5 of 27

1

Over two cycles of replication, a methylated C-G will be replaced with an?

Select one of the following:

  • A-T

  • C-C

  • G-T

Explanation

Question 6 of 27

1

What protein catalyses Direct Reversal of Guanine methylation?

Select one of the following:

  • Alkyl-Guanine Transferase

  • Adenosyl-Guanine Transferase

  • Adenyl-Guanine Transferase

Explanation

Question 7 of 27

1

The enzyme involved in direct reversal catalyses the transfer of CH3 to the enzyme, killing it

Select one of the following:

  • True
  • False

Explanation

Question 8 of 27

1

How does the Direct Reversal enzyme 'flip out' the damaged guanine?

Select one of the following:

  • A helix-helix motif

  • A helix-turn-helix motif

  • A sheet-turn-sheet motif

Explanation

Question 9 of 27

1

The enzyme for direct reversal is not specific and so works globally

Select one of the following:

  • True
  • False

Explanation

Question 10 of 27

1

What is the reading head of the Direct Reversal enzyme made of?

Select one of the following:

  • Ala & Gly

  • Ser & Val

  • Tyr & Ser

Explanation

Question 11 of 27

1

The enzyme binds to the major groove using an HTH motif

Select one of the following:

  • True
  • False

Explanation

Question 12 of 27

1

Which happens first in the CH3 transfer?

Select one of the following:

  • Lys pushes H onto Tyr

  • Lys pushes CH3 onto Tyr

  • Tyr pushes H onto Lys

Explanation

Question 13 of 27

1

The new H+ pushes e-....

Select one of the following:

  • Onto CH3

  • Onto O bound to CH3

  • Onto Lys bound to CH3

Explanation

Question 14 of 27

1

The e- added to the O pushes the CH3 onto the Lysine

Select one of the following:

  • True
  • False

Explanation

Question 15 of 27

1

Which of the following recognises the widowed G and fills the gap?

Select one of the following:

  • Arg on the head of the reading head

  • Lys on the head of the reading head

  • Val on the head of the reading head

Explanation

Question 16 of 27

1

UV irradiation of _ causes _?

Select one or more of the following:

  • Consecutive pyrimidines

  • Consecutive purines

  • Opens a 5-6 bond

  • Opens a 3-4 bond

  • Opens a 4-6 bond

Explanation

Question 17 of 27

1

A phosphobutane photo-dimer is formed

Select one of the following:

  • True
  • False

Explanation

Question 18 of 27

1

Why is UV radiation dangerous?

Select one or more of the following:

  • It alters DNA conformation

  • So helicases make mistakes

  • Bases are deleted

  • Bases are duplicated

Explanation

Question 19 of 27

1

How do 6-4 lesions form?

Select one of the following:

  • An O is transfered from on thymine to another

  • An N is transfered from on thymine to another

  • A C is transfered from on thymine to another

Explanation

Question 20 of 27

1

Damage is recognised by bases

Select one of the following:

  • True
  • False

Explanation

Question 21 of 27

1

How does photolyase work on 6-4 bonds?

Select one of the following:

  • Uses light to reverse the reaction

  • Uses UV to reverse the reaction

  • Binds to DNA and excises damaged bases

Explanation

Question 22 of 27

1

A 'protein wedge' forces DNA apart so it can work

Select one of the following:

  • True
  • False

Explanation

Question 23 of 27

1

An e- from diazaflavin is removed by UV and is passed onto what?

Select one of the following:

  • FADH

  • NAD

  • NADH

Explanation

Question 24 of 27

1

The electron will hopefully break the distorted bonds

Select one of the following:

  • True
  • False

Explanation

Question 25 of 27

1

The photolyase process can only work once

Select one of the following:

  • True
  • False

Explanation

Question 26 of 27

1

Which residues bind to the phosphate backbone in photolyase?

Select one or more of the following:

  • Glu

  • Arg

  • Gln

Explanation

Question 27 of 27

1

What does the widowed base pair bind to?

Select one of the following:

  • Water

  • Methyl

  • ATP

Explanation

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