Bacterial Metabolism & Industrial Microbiology

Question 1 of 33

1

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The chemical processes that occur within a living organism in order to maintain life is known as its

Explanation

Question 2 of 33

1

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Metabolism consists of two processes:
= Breakdown of chemical compounds via exergonic reactions
= Biosynthesis or chemical compounds via endergonic reactions

Explanation

Question 3 of 33

1

Exergonic reactions ❌
Endergonic reactions ❌

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require energy

release energy

Explanation

Question 4 of 33

1

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We have already established that organisms can be classified based on their energy source; Chemotrophs and Phototrophs. The Chemotroph category can be broken down into two further subcategories based on whether the organism utilises organic or inorganic substances:
( Chemoorganotrophs, Chemolithotrophs ) = Utilise organic compounds to obtain energy
( Chemolithotrophs, Chemoorganotrophs ) = Utilise inorganic compounds to obtain energy

Explanation

Question 5 of 33

1

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Different types of bacteria based on oxygen requirement:
1: aerobes need oxygen because they cannot ferment or respire anaerobically.
2: anaerobes are poisoned by oxygen.
3: anaerobes can grow with or without oxygen because they can metabolise energy aerobically or anaerobically.
4: need oxygen because they cannot ferment or respire anaerobically. However, they are poisoned by high concentrations of oxygen.
5: organisms do not require oxygen as they metabolise energy anaerobically. Unlike obligate anaerobes however, they are not poisoned by oxygen.

Explanation

Question 6 of 33

1

The difference between aerotolerant and obligate anaerobic bacteria is that whilst they both produce energy anaerobically aerotolerant bacteria are not poisoned by oxygen where obligate anaerobic bacteria are.

Select one of the following:

True
False

Explanation

Question 7 of 33

1

The difference between aerotolerant and obligate anaerobic bacteria is that whilst they both produce energy anaerobically aerotolerant bacteria are not poisoned by oxygen where obligate anaerobic bacteria are.

Select one of the following:

True
False

Explanation

Question 8 of 33

1

Match the mechanism of energy production to its description:
❌ = ATP is generated when a phosphorylated intermediate transfers its phosphate to ADP
❌ = ATP is synthesized during the transport of electrons along an electron transport chain to an electron acceptor
❌ = Similar to oxidative phosphorylation but electrons are provided by reduced chlorophyll molecules

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Photophosphorylation

Oxidative Phosphorylation

Substrate level phosphorylation

Explanation

Question 9 of 33

1

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Chemoorganotrophic bacteria obtain energy (ATP) from carbohydrates by which two basic processes? Alphabetical order.

Explanation

Question 10 of 33

1

How is the ATP produced in fermentation?

Select one of the following:

Substrate level phosphorylation
Oxidative phosphorylation
Photophosphorylation

Explanation

Question 11 of 33

1

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The Embden Meyerhof pathway is also known as and is the most common biochemical pathway for the fermentation of glucose.

Explanation

Question 12 of 33

1

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The process of glycolysis yields ( 2, 4, 6, 8 ) molecules of ATP

Explanation

Question 13 of 33

1

One of the main factors of fermentation is that NAD- and NADP+ must be reduced back into NADH and NADPH

Select one of the following:

True
False

Explanation

Question 14 of 33

1

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Yeast is grown is grown in two stages:
Firstly, an ( aerobic, anaerobic ) stage to cause yeast to grow and multiply
Secondly, an ( anaerobic, aerobic ) stage to cause yeast to produce energy via fermentation, thus producing ethanol as a by product.

Explanation

Question 15 of 33

1

Which type of fermentation occurs in E.coli?

Select one of the following:

Mixed acid fermentation
2,3-Butanediol Fermentation
Propionic Acid Fermentation
Butyric Acid Fermentation

Explanation

Question 16 of 33

1

Which of the following types of fermentation produces the holes seen in swiss cheese due to the release of CO2?

Select one of the following:

Propionic Fermentation
Butyric Acid Fermentation
2-3,Butanediol Fermentation
Mixed Acid Fermentation

Explanation

Question 17 of 33

1

Which of the following types of bacteria utilises Butyric Acid Fermentation?

Select one of the following:

Clostridium
Klebsiella
Enterobacter
Streptococcus
Lactobacillus

Explanation

Question 18 of 33

1

Different microbes produce different fermentation end products:
Lactic acid bacteria produce lactic acid which is important for the ❌
Propionic bacteria produce propionic acid, acetic acid and CO2 which is important for the ❌

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dairy industry (yoghurts, etc)

use in Swiss cheese

Explanation

Question 19 of 33

1

Bioethanol used in some modes of transport is typically produced by Saccharomyces Cerevisiae

Select one of the following:

True
False

Explanation

Question 20 of 33

1

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In ( yeasts, molds ), 10-20% of glucose is degraded via the Pentose Phosphate pathway

Explanation

Question 21 of 33

1

In terms of respiration, how is the ATP produced?

Select one of the following:

Substrate-level Phosphorylation
Oxidative Phosphorylation
Photophosphorylation

Explanation

Question 22 of 33

1

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There are two types of respiration:
Aerobic Respiration, whereby is the final electron acceptor and H20 is formed
Anaerobic Respiration, whereby other inorganic molecules, most commonly , is the terminal electron acceptor.

Explanation

Question 23 of 33

1

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In the TCA cycle of respiration, pyruvate is fully ( oxidised, reduced ) to CO2.

Explanation

Question 24 of 33

1

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The four carbon molecule which combines with Acetyl-Coenzyme A to become a six carbon molecule for the TCA cycle is called .
The six carbon molecule in question is called .

Explanation

Question 25 of 33

1

How many Acetyl Coenzyme A compounds are yielded from the Pyruvate produced at the end of glycolysis?

Select one of the following:

1
2
3
4

Explanation

Question 26 of 33

1

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The products of the TCA cycle are:
molecules of CO2
molecules of NADH
molecule of FADH

Explanation

Question 27 of 33

1

Electrons from the TCA cycle are transferred to the electron transport chain via which of the following?

Select one or more of the following:

CO2
NADH
FADH
GTP

Explanation

Question 28 of 33

1

Which of the following is also involved in fatty acid biosynthesis and can be produced directly from ketone bodies?

Select one of the following:

Acetyl CoA
Oxaloacetate
Succinyl-CoA
Alpha Ketoglutarate

Explanation

Question 29 of 33

1

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The generation of an electrochemical potential across a cell membrane via the trapping of H+ions on one side and OH- ions on the other in order to drive the electron transport chain is known as the force

Explanation

Question 30 of 33

1

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The proton motive force is the driving force for ATP synthesis via the membrane enzyme complex called

Explanation

Question 31 of 33

1

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The terminal electron acceptor in the electron transport chain is

Explanation

Question 32 of 33

1

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How many molecules is created in total by Aerobic respiration?

Explanation

Question 33 of 33

1

Anaerobic respiration is unique to prokaryotes (where terminal electron acceptors other than oxygen are used)

Select one of the following:

True
False

	Created by Matthew Coulson over 4 years ago

The first afternoon of the course - second lecture given.

Bacterial Metabolism & Industrial Microbiology

Question 1 of 33

Fill the blank space to complete the text.

The chemical processes that occur within a living organism in order to maintain life is known as its

Explanation

Question 2 of 33

Fill the blank spaces to complete the text.

Metabolism consists of two processes: = Breakdown of chemical compounds via exergonic reactions = Biosynthesis or chemical compounds via endergonic reactions

Explanation

Question 3 of 33

Exergonic reactions ❌ Endergonic reactions ❌

Drag and drop to complete the text.

Explanation

Question 4 of 33

Select from the dropdown lists to complete the text.

Explanation

Question 5 of 33

Fill the blank spaces to complete the text.

Explanation

Question 6 of 33

The difference between aerotolerant and obligate anaerobic bacteria is that whilst they both produce energy anaerobically aerotolerant bacteria are not poisoned by oxygen where obligate anaerobic bacteria are.

Select one of the following:

Explanation

Question 7 of 33

The difference between aerotolerant and obligate anaerobic bacteria is that whilst they both produce energy anaerobically aerotolerant bacteria are not poisoned by oxygen where obligate anaerobic bacteria are.

Select one of the following:

Explanation

Question 8 of 33

Drag and drop to complete the text.

Explanation

Question 9 of 33

Fill the blank spaces to complete the text.

Chemoorganotrophic bacteria obtain energy (ATP) from carbohydrates by which two basic processes? Alphabetical order.

Explanation

Question 10 of 33

How is the ATP produced in fermentation?

Select one of the following:

Explanation

Question 11 of 33

Fill the blank space to complete the text.

The Embden Meyerhof pathway is also known as and is the most common biochemical pathway for the fermentation of glucose.

Explanation

Question 12 of 33

Select from the dropdown list to complete the text.

The process of glycolysis yields 2 4 6 8( 2, 4, 6, 8 ) molecules of ATP

Explanation

Question 13 of 33

One of the main factors of fermentation is that NAD- and NADP+ must be reduced back into NADH and NADPH

Select one of the following:

Explanation

Question 14 of 33

Select from the dropdown lists to complete the text.

Yeast is grown is grown in two stages: Firstly, an aerobic anaerobic( aerobic, anaerobic ) stage to cause yeast to grow and multiply Secondly, an anaerobic aerobic( anaerobic, aerobic ) stage to cause yeast to produce energy via fermentation, thus producing ethanol as a by product.

Explanation

Question 15 of 33

Which type of fermentation occurs in E.coli?

Select one of the following:

Explanation

Question 16 of 33

Which of the following types of fermentation produces the holes seen in swiss cheese due to the release of CO2?

Select one of the following:

Explanation

Question 17 of 33

Which of the following types of bacteria utilises Butyric Acid Fermentation?

Select one of the following:

Explanation

Question 18 of 33

Different microbes produce different fermentation end products: Lactic acid bacteria produce lactic acid which is important for the ❌ Propionic bacteria produce propionic acid, acetic acid and CO2 which is important for the ❌

Drag and drop to complete the text.

Explanation

Question 19 of 33

Bioethanol used in some modes of transport is typically produced by Saccharomyces Cerevisiae

Select one of the following:

Explanation

Question 20 of 33

Select from the dropdown list to complete the text.

In yeasts molds( yeasts, molds ), 10-20% of glucose is degraded via the Pentose Phosphate pathway

Explanation

Question 21 of 33

Metabolism consists of two processes:
= Breakdown of chemical compounds via exergonic reactions
= Biosynthesis or chemical compounds via endergonic reactions

Exergonic reactions ❌
Endergonic reactions ❌

The process of glycolysis yields ( 2, 4, 6, 8 ) molecules of ATP

Yeast is grown is grown in two stages:
Firstly, an ( aerobic, anaerobic ) stage to cause yeast to grow and multiply
Secondly, an ( anaerobic, aerobic ) stage to cause yeast to produce energy via fermentation, thus producing ethanol as a by product.

Different microbes produce different fermentation end products:
Lactic acid bacteria produce lactic acid which is important for the ❌
Propionic bacteria produce propionic acid, acetic acid and CO2 which is important for the ❌

In ( yeasts, molds ), 10-20% of glucose is degraded via the Pentose Phosphate pathway

There are two types of respiration:
Aerobic Respiration, whereby is the final electron acceptor and H20 is formed
Anaerobic Respiration, whereby other inorganic molecules, most commonly , is the terminal electron acceptor.

In the TCA cycle of respiration, pyruvate is fully ( oxidised, reduced ) to CO2.

The four carbon molecule which combines with Acetyl-Coenzyme A to become a six carbon molecule for the TCA cycle is called .
The six carbon molecule in question is called .

The products of the TCA cycle are:
molecules of CO2
molecules of NADH
molecule of FADH