- Scientists can use tools that cut, separate, then replicate DNA base by base to read DNA sequences from any cell

Cutting DNA:

- Difference of nucleic acids to other macro-molecules

- Molecules of DNA too large for analyzing

- Need to be cut into smaller pieces

Restriction Enzymes: Enzymes produced by bacteria that cut DNA into precise pieces (restriction fragments)

- Many, with each one cutting at a different nucleotide sequence

- EcoRI restriction enzyme recognizes only the base sequence GAATTC

- Cuts between G and A bases leaving AATT

Sticky ends - Overhangs left by restriction enzymes that can "stick" or bond to complementary sequences (AATT is sticky end)

Blunt ends - Straight ends left by a restriction enzyme that cannot bond onto a complementary sequence

	Created by Shelby Conroy about 5 years ago

Preparation for exam, covering all sections of Chapters 14 in the Miller Levine Biology Textbook (Still in progress)