Where does protein synthesis occur and what intermediate are synthesised to allow this process to occur? (3)

What is meant by "Coding strand"? (2)

What is meant by "Non-coding strand (2)

What is RNA Polymerase?

What is a promoter?

What is a terminator?

Which strand of a DNA molecule can act as the coding strand?

What are the requirements of RNA Polymerase?(3)

Which NTP releases the most energy when the 2-3 phosphate bond is hydrolysed?

How does RNA Polymerisation differ from DNA polymerisation?

Describe the first Stage of Transcription (4)

Describe the second Stage of Transcription (3)

Describe the third Stage of Transcription (2)

What do promoter sequences signify? (2)

How is a promoter sequence identified? (2)

Name 2 common promoter sequences, how the one sequence got its name, and their function (4)

How are different RNA molecules synthesised by E. Coli?

How many RNA Polymerase forms exist in E. Coli?
What are these forms?(4)

What are the functions of these 2 forms?

Draw an annotated diagram showing the organisation and transcription of a simple bacterial protein-coding gene (11)

Describe the initiation of bacterial transcription (5)

Describe the elongation process of bacterial transcription (4)

Describe the termination process of bacterial transcription

What essential ion is found at the active site of polymerase enzymes?

What is different about the DNA leaving the active site of a polymerase as opposed to DNA entering the active site?

How is eukaryotic transcription different to prokaryotic transcription? (5)

What are the names of the eukaryotic RNA Polymerases and the product they form? (3)

Which type of RNA is the most abundant and which is the most scarce in the cell?

Draw a diagram showing the splicing of coding DNA by eukaryotic polymerases

Describe the process of DNA splicing